CAJ | 학술논문

研究了Pb-Zn-Sn黄铜矿精矿在混合中度嗜热微生物槽浸过程中的细菌群落结构变化，并监测浸出体系中金属离子浓度、溶液电位、溶液pH值变化，通过聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)技术分析微生物群落的结构变化。结果表明，最终铜浸出率高达85.6%，在浸出前期，Acidithiobacillus caldus为优势群落，从第18天开始到浸出结束，Sulfobacillus thermosulfidooxidans为优势群落，但Leptospirillum ferriphilum丰度变化较小。试验结果表明，适当较高的溶液电位和合适的铁离子浓度对黄铜矿精矿的生物浸出作用很关键。
연구료Pb-Zn-Sn황동광정광재혼합중도기열미생물조침과정중적세균군락결구변화，병감측침출체계중금속리자농도、용액전위、용액pH치변화，통과취합매련식반응-한제성편단장도다태성(PCR-RFLP)기술분석미생물군락적결구변화。결과표명，최종동침출솔고체85.6%，재침출전기，Acidithiobacillus caldus위우세군락，종제18천개시도침출결속，Sulfobacillus thermosulfidooxidans위우세군락，단Leptospirillum ferriphilum봉도변화교소。시험결과표명，괄당교고적용액전위화합괄적철리자농도대황동광정광적생물침출작용흔관건。
The variation of microbial community structure was investigated for the tank bioleaching process of Pb-Zn-Sn chalcopyrite concentrate in the presence of mixed moderately thermophilic bacteria. The parameters, such as pH value, solution potential and concentrations of metal ions, were determined by the method of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to analyze the succession of microbial community. The results showed that a final copper extraction rate of 85.6% could be obtained after tank bioleaching for 30 d. The Acidithiobacillus caldus was the dominant population with abundance of about 73.80%in the initial stage, then Sulfobacillus thermosulfidooxidans dominated from the 18th day to the end of bioleaching, while the abundance of Leptospirillum ferriphilum changed slightly. A higher solution potential within a certain range and appropriate concentration of ferric ions were essential for this tank bioleaching of chalcopyrite.