CAJ | 학술논문

本实验对患病鸭绿江斑鳜进行病毒检测，将表现典型真鲷虹彩病毒（RSIV）病症状的斑鳜鱼肾脏和脾脏研磨成组织匀浆，采用试剂盒方法提取病毒DNA，并应用PCR 方法对两对引物进行扩增获得570 bp和568 bp两条基因片段，将其中的568 bp基因片段连接至pMD18-T 载体中，经酶切鉴定后序列分析表明，与GenBank 登录的RSIV 同源性均为99.9%，命名为RSIV-DD。表明本研究在国内首次从淡水鱼斑鳜中分离到RSIV。
본실험대환병압록강반궐진행병독검측，장표현전형진조홍채병독（RSIV）병증상적반궐어신장화비장연마성조직균장，채용시제합방법제취병독DNA，병응용PCR 방법대량대인물진행확증획득570 bp화568 bp량조기인편단，장기중적568 bp기인편단련접지pMD18-T 재체중，경매절감정후서렬분석표명，여GenBank 등록적RSIV 동원성균위99.9%，명명위RSIV-DD。표명본연구재국내수차종담수어반궐중분리도RSIV。
Kidneys and livers were collected from the diseased Yalu River mandarinfish suffered from typical red sea bream iridoviral disease, and grinded into tissue homogenate and the virus DNA was extracted by the kit method and detected by using PCR method. Two gene fragments of 570 bp and 568 bp DNA were amplified by PCR method and the 568 bp DNA was cloned into pMD18-T vector. Sequencing result of the 568 bp DNA indicated that the sequence of nucleotide acid homology was 99.9%with the reference strains of RSIV in GenBank and it was named RSIV-DD. The result demonstrated that the RSIV was isolated for the first time from the naturally infected mandarinfish in China.