CAJ | 학술논문

目的：研究螺内酯对人急性白血病细胞Jurkat体外增殖的抑制及诱导凋亡的作用。方法将终浓度分别是10、50及100μmol/L的螺内酯加入Jurkat细胞的培养体系中,24 h内每隔4 h通过 MTT法分析Jur-kat细胞的增殖抑制率,Annexin V/PI流式细胞术法分析Jurkat细胞的早期凋亡率。结果螺内酯与Jurkat细胞共同培养12 h后,螺内酯能显著增加对细胞的增殖抑制作用,并且与药物浓度成正相关,各浓度组的抑制率随着培养时间的延长而升高,与药物干预时间成正相关；螺内酯处理Jurkat细胞24 h,各浓度组诱导细胞凋亡率分别为：10μmol/L组(11．2±0．35)%、50μmol/L组(29．8±1．27)%及100μmol/L(56．5±1．41)%,各个浓度组诱导细胞凋亡率与药物浓度成正相关。结论螺内酯对人T淋巴细胞白血病Jurkat细胞具有抑制增殖和诱导凋亡的作用,提示该药可能具有潜在抑瘤作用。
목적：연구라내지대인급성백혈병세포Jurkat체외증식적억제급유도조망적작용。방법장종농도분별시10、50급100μmol/L적라내지가입Jurkat세포적배양체계중,24 h내매격4 h통과 MTT법분석Jur-kat세포적증식억제솔,Annexin V/PI류식세포술법분석Jurkat세포적조기조망솔。결과라내지여Jurkat세포공동배양12 h후,라내지능현저증가대세포적증식억제작용,병차여약물농도성정상관,각농도조적억제솔수착배양시간적연장이승고,여약물간예시간성정상관；라내지처리Jurkat세포24 h,각농도조유도세포조망솔분별위：10μmol/L조(11．2±0．35)%、50μmol/L조(29．8±1．27)%급100μmol/L(56．5±1．41)%,각개농도조유도세포조망솔여약물농도성정상관。결론라내지대인T림파세포백혈병Jurkat세포구유억제증식화유도조망적작용,제시해약가능구유잠재억류작용。
Objective To study the proliferated inhibition and apoptosis of human T-acute lymphoblastic leu-kemia cells lines Jurkat treated with spironolactone. Methods Jurkat cells were treated with different concentrations of spironolactone (10,50,100 μmol/L) in culture. MTT assay was used to observe the proliferated inhibition rate of Jur-kat at 4 hours interval and the flow cytometry was applied to analyze Annexin V/PI apoptosis rate of Jurkat. Results 12 hours after treatment,spironolactone could obviously inhibit the proliferation of Jurkat and the effect had dose and treated time dependence. 24 hours after treatment, apoptosis of these concentrations were ( 11. 2 ± 0. 35 )%, ( 29. 8 ± 1. 27)%,(56. 5 ± 1. 41)%,and the apoptosis rate increased in dose dependence. Conclusion Spironolactone can in-hibit the proliferation and induce apoptosis of Jurkat,it suggests that this medicine may have the effect of tumor inhibi-tion.