CAJ | 학술논문

目的：评价多粘菌素B与美罗培南体外联合应用对临床分离的30株泛耐药铜绿假单胞菌的抗菌效应。方法采用微量肉汤稀释法和棋盘法测定多粘菌素B与美罗培南单独用药和联合用药后，临床分离的30株泛耐药的铜绿假单胞菌的最小抑菌浓度（minimal inhibitory concentration，MIC），及计算部分抑菌浓度指数（fractional inhibitory concentration，FIC），根据FIC指数判定联合效应类型，判断是否有协同或拮抗作用。结果多粘菌素B与美罗培南联用后，药物对泛耐药铜绿假单胞菌的MIC值均显著降低，FIC指数在0～0.5百分率为60%；FIC指数在0.5～1.0的百分率为30%。结论多粘菌素B与美罗培南联合用药后，以协同作用和相加作用为主，测定出分离的30株泛耐药铜绿假单胞菌的抗菌作用无拮抗作用。
목적：평개다점균소B여미라배남체외연합응용대림상분리적30주범내약동록가단포균적항균효응。방법채용미량육탕희석법화기반법측정다점균소B여미라배남단독용약화연합용약후，림상분리적30주범내약적동록가단포균적최소억균농도（minimal inhibitory concentration，MIC），급계산부분억균농도지수（fractional inhibitory concentration，FIC），근거FIC지수판정연합효응류형，판단시부유협동혹길항작용。결과다점균소B여미라배남련용후，약물대범내약동록가단포균적MIC치균현저강저，FIC지수재0～0.5백분솔위60%；FIC지수재0.5～1.0적백분솔위30%。결론다점균소B여미라배남연합용약후，이협동작용화상가작용위주，측정출분리적30주범내약동록가단포균적항균작용무길항작용。
Objective To evaluate the anti-bacteria effects of polymyxin B combined with meropenem against 30 strains pan-drug resistant pseudomonas aeruginosa that separated in clinic. Methods The minimal inhibitory concentration (MICs) of 30 strains pan-drug resistant pseudomonas aeruginosa after treated with polymyxin B and meropenem as single-use or combination use were determined by both microdilution method and checkerboard method. The FIC index was calculated, then the type of combination effect was determined according to FIC index, which was used to determine whether there was synergistic or antagonistic effects. Results The MICs of pan-drug resistant pseudomonas aeruginosa were reduced significantly after polymyxin B combined with meropenem when compared with single-use. The percentages of the FIC index that less than 0.5 and the index between 0.5 and 1 were 60%and 30%respectively. Conclusion The results indicate that the combinations of polymyxin B with meropenem have good synergistic and additive effects against pan-drug resistant pseudomonas aeruginosa, there is no antagonism.