CAJ | 학술논문

目的：建立正斜丸的质量控制方法。方法：采用薄层层析法鉴别正斜丸中黄芪、白附子、防风、秦艽，采用高效液相色谱法-蒸发光散射检测器法对正斜丸中黄芪甲苷含量进行测定。色谱柱为Kromosil C18柱（4.6mm×250mm，5μm），流动相为乙腈：水（34∶66），流速为1.0ml/min。蒸发光散射检测器参数为：飘逸管温度100.8℃，N2流速为2.7L/min。结果：用薄层色谱法可鉴别出正斜丸中黄芪、白附子、防风、秦艽的特征斑点，阴性对照无干扰，专属性强。黄芪甲苷在50.12～300.7μg/ml范围呈较好线性关系，r=0.9995，平均加样回收率为98.42%，RSD为1.09%（n=6）。结论：本文建立的方法准确可靠，重现性好，能有效控制正斜丸的质量。
목적：건립정사환적질량공제방법。방법：채용박층층석법감별정사환중황기、백부자、방풍、진구，채용고효액상색보법-증발광산사검측기법대정사환중황기갑감함량진행측정。색보주위Kromosil C18주（4.6mm×250mm，5μm），류동상위을정：수（34∶66），류속위1.0ml/min。증발광산사검측기삼수위：표일관온도100.8℃，N2류속위2.7L/min。결과：용박층색보법가감별출정사환중황기、백부자、방풍、진구적특정반점，음성대조무간우，전속성강。황기갑감재50.12～300.7μg/ml범위정교호선성관계，r=0.9995，평균가양회수솔위98.42%，RSD위1.09%（n=6）。결론：본문건립적방법준학가고，중현성호，능유효공제정사환적질량。
Objective: To establish the quality method for Zhengxie pill. Methods: TLC was used for the identification and HPLC-ELSD was used for the determination of astragaloside glycoside content in the Zhengxie pills. Kromosil C18 column (4.6mm×250 mm, 5μm)was used;Acetonitrile:H2O (34:66) was used as a mobile phase;Flow rate was 1.0ml/min;Detection wavelength was 358nm. ELSD parameters:elegant tube temperature was 100.8℃, the flow rate of N2 was 2.7L/min. Results:The characteristic spots of astragalus, white monkshood, windbreak and gentiana could be identified, yet without interference of negative control. Astragaloside glycoside showed good linear relationship in 50.12~300.7μg/ml (r=0.9995), the average recovery was 98.42%, RSD was 1.09%(n=6). Conclusion:The method showed good precision and reproducibility, the result is accurate, which can be used for quality standard for Zhengxie pill.