中国生化药物杂志
中國生化藥物雜誌
중국생화약물잡지
CHINESE JOURNAL OF BIOCHEMICAL PHARMACEUTICS
2014年
2期
33-36
,共4页
文红波%曹运长%虞佳%王五洲
文紅波%曹運長%虞佳%王五洲
문홍파%조운장%우가%왕오주
白杨素%HepG2细胞%细胞分化%黄酮类化合物
白楊素%HepG2細胞%細胞分化%黃酮類化閤物
백양소%HepG2세포%세포분화%황동류화합물
chrysin%HepG 2 cells%cell differentiation%lfavonoid compounds
目的：探讨白杨素对人肝癌HepG2细胞的诱导分化和凋亡作用。方法体外培养人肝癌HepG2细胞，以全反式维甲酸为阳性对照，用白杨素干预细胞，台盼蓝计数法和MTT法检测药物对细胞增殖活力的影响；瑞氏-姬姆萨和考马斯亮蓝染色观察细胞核质比和微管微丝排列的变化；放射免疫法检测细胞甲胎蛋白（Alpha-fetoprotein，AFP）的分泌量；酶促反应试剂盒检测细胞中γ-谷胺酰转肽酶（γ-glutamyltranspeptidase，γ-GT）和碱性磷酸酶（alkaline phosephatase，ALP）的活性；Diamondstone分光光度法测定细胞中酪氨酸α-酮戊二酸转氨酶（tyrosine-α-ketoglutaric acid transaminase，TAT）的合成情况。结果1～100μmol/L白杨素和全反式维甲酸处理HepG2细胞48 h后，能显著抑制肝癌细胞的增殖（P＜0.05，P＜0.01），2种药物对细胞增殖的抑制效价相当并存在量效关系。10μmol/L药物作用48 h，细胞的形态和微管微丝排列由肿瘤细胞向成熟细胞分化；药物处理24～96 h后，细胞AFP的分泌量和γ-GT的活性明显降低，ALP和TAT的活性则显著升高（P＜0.05，P＜0.01）。结论白杨素具有抑制人肝癌HepG2细胞增殖并诱导其向正常细胞分化的作用。
目的：探討白楊素對人肝癌HepG2細胞的誘導分化和凋亡作用。方法體外培養人肝癌HepG2細胞，以全反式維甲痠為暘性對照，用白楊素榦預細胞，檯盼藍計數法和MTT法檢測藥物對細胞增殖活力的影響；瑞氏-姬姆薩和攷馬斯亮藍染色觀察細胞覈質比和微管微絲排列的變化；放射免疫法檢測細胞甲胎蛋白（Alpha-fetoprotein，AFP）的分泌量；酶促反應試劑盒檢測細胞中γ-穀胺酰轉肽酶（γ-glutamyltranspeptidase，γ-GT）和堿性燐痠酶（alkaline phosephatase，ALP）的活性；Diamondstone分光光度法測定細胞中酪氨痠α-酮戊二痠轉氨酶（tyrosine-α-ketoglutaric acid transaminase，TAT）的閤成情況。結果1～100μmol/L白楊素和全反式維甲痠處理HepG2細胞48 h後，能顯著抑製肝癌細胞的增殖（P＜0.05，P＜0.01），2種藥物對細胞增殖的抑製效價相噹併存在量效關繫。10μmol/L藥物作用48 h，細胞的形態和微管微絲排列由腫瘤細胞嚮成熟細胞分化；藥物處理24～96 h後，細胞AFP的分泌量和γ-GT的活性明顯降低，ALP和TAT的活性則顯著升高（P＜0.05，P＜0.01）。結論白楊素具有抑製人肝癌HepG2細胞增殖併誘導其嚮正常細胞分化的作用。
목적：탐토백양소대인간암HepG2세포적유도분화화조망작용。방법체외배양인간암HepG2세포，이전반식유갑산위양성대조，용백양소간예세포，태반람계수법화MTT법검측약물대세포증식활력적영향；서씨-희모살화고마사량람염색관찰세포핵질비화미관미사배렬적변화；방사면역법검측세포갑태단백（Alpha-fetoprotein，AFP）적분비량；매촉반응시제합검측세포중γ-곡알선전태매（γ-glutamyltranspeptidase，γ-GT）화감성린산매（alkaline phosephatase，ALP）적활성；Diamondstone분광광도법측정세포중락안산α-동무이산전안매（tyrosine-α-ketoglutaric acid transaminase，TAT）적합성정황。결과1～100μmol/L백양소화전반식유갑산처리HepG2세포48 h후，능현저억제간암세포적증식（P＜0.05，P＜0.01），2충약물대세포증식적억제효개상당병존재량효관계。10μmol/L약물작용48 h，세포적형태화미관미사배렬유종류세포향성숙세포분화；약물처리24～96 h후，세포AFP적분비량화γ-GT적활성명현강저，ALP화TAT적활성칙현저승고（P＜0.05，P＜0.01）。결론백양소구유억제인간암HepG2세포증식병유도기향정상세포분화적작용。
Objective To investigate the effects of chrysin(ChR) on the induction of differentiation and apoptosis-promoting of HepG 2 human primary hepatocacinoma cells. Methods The HepG 2 cells were cultured in vitro, and then treated with ChR and all-trans retinotic Acid (RA), respectively, the alterations of nucleocytoplasm and tubulin arrangement after Gimsa staining and Coomassie brilliant blue staining were observed. The survival rate and the inhibitory rates of HepG 2 cells were determine by trypan blue counting method and MTT assay. The Alpha-fetoprotein(AFP) secretory amounts of the cells were detected by radioimmunoassay(RIA). The activities of alkaline phosphatase(ALP) andγ-glutamyltranspeptidase(γ-GT) were assayed by enzymatic reaction kit. The synthesis of tyrosine-α-ketoglutaric acid transaminase(TAT) in cells were investigated by Diamondstone spectrophotometry. Results After treatment with ChR or RA at 1.0~100μmol/L for 48 h, the proliferation of HepG 2 cells were inhibited significantly, compared with vehicle group (P<0.05 or P<0.01), the inhibitory potency of both ChR and RA on HepG 2 cells was equivalent and indicated in dose-dependent manner. After treatment with 10μmol/L ChR or RA for 48 h, HepG 2 cells disaggregated and grew to spindle-shape, their nuclei became smaller and the number of nucleolus were fewer. Furthermore, tubulin arrangement of cells tended to be more ordered and the tubulin synthesis increased significantly. At 24~96 hours treated with 10μmol/L ChR, the activities of TAT and ALP in cells were all increased distinctly (P<0.05, P<0.01), and the secretory amounts of AFP and the specific activities ofγ-GT were decreased significantly (P<0.05, P<0.01). Conclusion Chrysin can inhibit the proliferation of HepG 2 cells and induce them to differentiate to mature cells.
