南京晓庄学院学报
南京曉莊學院學報
남경효장학원학보
JOURNAL OF NANJING XIAOZHUANG COLLEGE
2012年
3期
76-79
,共4页
红酵母%类胡萝卜素%培养条件
紅酵母%類鬍蘿蔔素%培養條件
홍효모%류호라복소%배양조건
Rhodotorula sp.%carotenoid%culture conditions
对诱变红酵母RY-09菌株生物合成类胡萝卜素的培养基组成和培养条件进行初步探索.通过生长曲线的测定和各种发酵条件的优化,确定最佳培养条件：初始pH值4.5、培养温度26℃、培养时间64 h;在最优条件下,红酵母生物量为9.03 g·100 mL-1、菌体生物合成类胡萝卜素最大量为5.46 mg·L-1.同时采用HPLC法初步确定类胡萝卜素的主要组成.
對誘變紅酵母RY-09菌株生物閤成類鬍蘿蔔素的培養基組成和培養條件進行初步探索.通過生長麯線的測定和各種髮酵條件的優化,確定最佳培養條件：初始pH值4.5、培養溫度26℃、培養時間64 h;在最優條件下,紅酵母生物量為9.03 g·100 mL-1、菌體生物閤成類鬍蘿蔔素最大量為5.46 mg·L-1.同時採用HPLC法初步確定類鬍蘿蔔素的主要組成.
대유변홍효모RY-09균주생물합성류호라복소적배양기조성화배양조건진행초보탐색.통과생장곡선적측정화각충발효조건적우화,학정최가배양조건：초시pH치4.5、배양온도26℃、배양시간64 h;재최우조건하,홍효모생물량위9.03 g·100 mL-1、균체생물합성류호라복소최대량위5.46 mg·L-1.동시채용HPLC법초보학정류호라복소적주요조성.
The paper deals with the culture medium compositions and fermentation conditions of biosynthesis of carotenoids from rhodotorula RY-09 strain.Through the determination of the growth curve and the optimization of fermentation conditions,some main parameters were selected,with the initial pH at 4.5,the temperature at 26 ℃ and the fermentation time set for 64 hours.The composition of carotenoids was mostly detected by HPLC.The cell of rhodotorula RY-09 and the content of carotenoids were 9.03 g/100 mL and 5.46 mg·L-1 respectively.
