喀什师范学院学报
喀什師範學院學報
객십사범학원학보
JOURNAL OF KASHGAR TEACHERS COLLEGE
2012年
3期
56-59
,共4页
牛肌腱%酸一酶结合法%胶原纤维%得率
牛肌腱%痠一酶結閤法%膠原纖維%得率
우기건%산일매결합법%효원섬유%득솔
Bovine tendon%Acid - enzyme%collagen fibers%Yield
以牛肌腱为原料，分别利用酸一酶结合法和酶法从冻干碎块中提取胶原纤维，经过盐析、透析、离心和冷冻干燥后制备得到高纯度胶原纤维．通过测定胶原纤维中羟脯氨酸的含量，得到酸一酶结合法的得率为44．9％，远大于单独用酶提取胶原纤维的得率33．8％．对制备的胶原纤维进行红外扫描和差示量热扫描（DSC），结果表明胶原纤维保持了完整的三股螺旋结构，DSC扫描结果表明冻干胶原纤维的变性温度为60．8~C．
以牛肌腱為原料，分彆利用痠一酶結閤法和酶法從凍榦碎塊中提取膠原纖維，經過鹽析、透析、離心和冷凍榦燥後製備得到高純度膠原纖維．通過測定膠原纖維中羥脯氨痠的含量，得到痠一酶結閤法的得率為44．9％，遠大于單獨用酶提取膠原纖維的得率33．8％．對製備的膠原纖維進行紅外掃描和差示量熱掃描（DSC），結果錶明膠原纖維保持瞭完整的三股螺鏇結構，DSC掃描結果錶明凍榦膠原纖維的變性溫度為60．8~C．
이우기건위원료，분별이용산일매결합법화매법종동간쇄괴중제취효원섬유，경과염석、투석、리심화냉동간조후제비득도고순도효원섬유．통과측정효원섬유중간포안산적함량，득도산일매결합법적득솔위44．9％，원대우단독용매제취효원섬유적득솔33．8％．대제비적효원섬유진행홍외소묘화차시량열소묘（DSC），결과표명효원섬유보지료완정적삼고라선결구，DSC소묘결과표명동간효원섬유적변성온도위60．8~C．
The high purity collagen fibers was obtained after salting out, dialysis, centrifugation and freeze-dried. Through determining the hydroxyproline content of the collagen fibers, it was found that the collagen fibers of the Acid - enzyme binding with 44.9% much higher than that of enzymatic alone with33.8%. The results of the collagen fibers infrared scanning and Differential Scanning Calorimetry （DSC） showed that the collagen fibers was provided with the complete triple helix structure. And the DSC scan results showed that the denaturation temperature of the freeze-dried collagen fibers was 60℃.
