CAJ | 학술논문

[目的]探求快速、灵敏、可靠的检测食品中大肠杆菌的方法。[方法]通过选择性鉴定试验、敏感性试验及其在食品检测中的应用效果对大肠杆菌的4种检测方法：GB/T4789.38-2008第一法、SN/T1896-2007第二法、GB/T4789.38-2008第三法与COLI ID显色培养基法进行比较研究。[结果]4种方法检测对大肠杆菌选择性良好,SN/T1896-2007第二法敏感性较GB/T4789.38-2008第一法稍高,GB/T4789.38-2008第三法与COLI ID显色培养基法敏感性相差不大;4种方法应用在食品检测中,大肠杆菌阳性检出率无显著差异。[结论]SN/T1896-2007第二法、GB/T4789.38-2008第三法与COLI ID显色培养基法耗费比GB/T4789.38-2008第一法高,但检测周期短,可节省人力,具有快速、方便的优点,在日常检测中值得推广应用。
[목적]탐구쾌속、령민、가고적검측식품중대장간균적방법。[방법]통과선택성감정시험、민감성시험급기재식품검측중적응용효과대대장간균적4충검측방법：GB/T4789.38-2008제일법、SN/T1896-2007제이법、GB/T4789.38-2008제삼법여COLI ID현색배양기법진행비교연구。[결과]4충방법검측대대장간균선택성량호,SN/T1896-2007제이법민감성교GB/T4789.38-2008제일법초고,GB/T4789.38-2008제삼법여COLI ID현색배양기법민감성상차불대;4충방법응용재식품검측중,대장간균양성검출솔무현저차이。[결론]SN/T1896-2007제이법、GB/T4789.38-2008제삼법여COLI ID현색배양기법모비비GB/T4789.38-2008제일법고,단검측주기단,가절성인력,구유쾌속、방편적우점,재일상검측중치득추엄응용。
A rapid sensitive and reliable method for detecting Escherichia coli in food was explored.In this research,four methods,GB/T4789.38-2008,SN/T1896-2007,GB/T4789.38-2008 and COLI ID were compared with selective identification test,sensitivity test and effects of application in food examination.These four methods all had good selectivity for Escherichia coli.The second method of SN/T1896-2007 was slightly more sensitive than the first method of GB/T4789.38-2008.The third method of GB/T4789.38-2008 and COLI ID method didn＇t have distinct difference in sensitivity.The positive rates of these four methods showed no significant difference in detection of Escherichia coli.Although the costs of the second method of SN/T1896-2007,the third method of GB/T4789.38-2008 and COLI ID method are higher than the first method of GB/T4789.38-2008,the detection time of the former three methods can be shorter than that of the latter.In addition,Concerning their superiority of labor saving and convenience in use,the former three methods are worthy of recommending in routine food examination.