CAJ | 학술논문

巴斯德毕赤酵母（Pichiapastoris）表达系统是近20年来应用最广泛的真核表达系统之一，已有500多种蛋白在该系统得到了成功表达，然而在毕赤酵母基因组中却缺少蔗糖酶基因，因此无法利用蔗糖。本研究从酿酒酵母基因组中克隆得到蔗糖酶基因SUC2。将SUC2亚克隆后得到表达载体pPICZαA—SUC2。经电转化后，表达载体整合进入毕赤酵母KM71H基因组，得到能够利用蔗糖的毕赤酵母工程菌。
파사덕필적효모（Pichiapastoris）표체계통시근20년래응용최엄범적진핵표체계통지일，이유500다충단백재해계통득도료성공표체，연이재필적효모기인조중각결소자당매기인，인차무법이용자당。본연구종양주효모기인조중극륭득도자당매기인SUC2。장SUC2아극륭후득도표체재체pPICZαA—SUC2。경전전화후，표체재체정합진입필적효모KM71H기인조，득도능구이용자당적필적효모공정균。
Pichiapastoris is one of the most frequently used expression systems during the last two decades, and more than 500 different kinds of proteins have been successfully expressed. But Pichia pastoris is unable to utilize sucrose because its lack of invertase gene. In this study, the gene SUC2 encoding invertase was cloned from Saccharomyces cerevisiae chromosome. SUC2 was then subcloned into the vector pPICZaA to construct the recombinant pPICZaA-SUC2. The recombinant plasmid to construct the engineering strain utilizing sucrose. was then transformed into Pichia pastoris KM71H