北京联合大学学报：自然科学版
北京聯閤大學學報：自然科學版
북경연합대학학보：자연과학판
Journal of Beijing Union University
2012年
3期
41-45
,共5页
鼠尾藻%18S%rDNA%系统发生
鼠尾藻%18S%rDNA%繫統髮生
서미조%18S%rDNA%계통발생
Sargassum thunbergii%SSU rDNA%Phylogeny
通过制备野生鼠尾藻基因组DNA,用同源克隆技术获得鼠尾藻的SSU rDNA序列。该序列长度为1 784 bp,A、G、T、C 4种碱基的含量分别为24.66%、27.75%、26.35%和21.24%,序列已提交至GenBank,登录号为JN604979。选用GenBank数据库中已有的褐藻SSU rDNA序列构建系统发生树,结果显示：鼠尾藻与半叶马尾藻,铜藻以及Sargassum macrocarpum聚为一支,并且与半叶马尾藻的亲缘关系最近。
通過製備野生鼠尾藻基因組DNA,用同源剋隆技術穫得鼠尾藻的SSU rDNA序列。該序列長度為1 784 bp,A、G、T、C 4種堿基的含量分彆為24.66%、27.75%、26.35%和21.24%,序列已提交至GenBank,登錄號為JN604979。選用GenBank數據庫中已有的褐藻SSU rDNA序列構建繫統髮生樹,結果顯示：鼠尾藻與半葉馬尾藻,銅藻以及Sargassum macrocarpum聚為一支,併且與半葉馬尾藻的親緣關繫最近。
통과제비야생서미조기인조DNA,용동원극륭기술획득서미조적SSU rDNA서렬。해서렬장도위1 784 bp,A、G、T、C 4충감기적함량분별위24.66%、27.75%、26.35%화21.24%,서렬이제교지GenBank,등록호위JN604979。선용GenBank수거고중이유적갈조SSU rDNA서렬구건계통발생수,결과현시：서미조여반협마미조,동조이급Sargassum macrocarpum취위일지,병차여반협마미조적친연관계최근。
Genomic DNA of Sargassum thunbergii was extracted and the SSU rDNA sequence was amplified via PCR technique. The sequence results showed that it was composed 1784 nucleartides in which the A, G, T and C contents are 24, 66%, 27.75%, 26.35% and 21.24%, respectively. The sequence has been submitted to GenBank database, and the accesssion number is JN604979. Neighbor-joining phylogenetic trees based on SSU sequences are constructed and S. thunbergii was clustered with Sargassum confusum, Sargassum macrocarpum and Sargassum horneri. S. thunbergii exhibited a very close relationship to S. confusum.
