安徽科技学院学报
安徽科技學院學報
안휘과기학원학보
JOURNAL OF ANHUI SCIENCE AND TECHNOLOGY UNIVERSITY
2012年
3期
27-33
,共7页
霍山石斛%工厂化繁育%组培
霍山石斛%工廠化繁育%組培
곽산석곡%공엄화번육%조배
Dendrobium huoshanense%Factory breeding%Tissue culture
以野生霍山石斛种子为外植体,对霍山石斛组培快繁技术进行研究,旨在为霍山石斛规模化、标准化、产业化生产提供依据。结果表明,种子萌发与原球茎诱导最适培养基为MS+6-BA0.1mg/L+NAA0.5mg/L+20%土豆汁;原球茎继代增殖与分化最适培养基为1/2MS+6-BA0.5mg/L+NAA0.1mg/L+20%土豆汁;丛生芽增殖最适培养基为1/2MS+6-BA0.5mg/L+NAA0.5mg/L+20%土豆汁;壮苗生根最适培养基为1/2MS+NAA0.5mg/L+20%土豆汁;炼苗最适温度、光照及时间:温度(24±2)℃,光照6000Lx,炼苗时间10d,后3d将瓶盖打开;试管苗移栽最适基质配方:草碳根+碎木屑+碎石(1:1:1)。
以野生霍山石斛種子為外植體,對霍山石斛組培快繁技術進行研究,旨在為霍山石斛規模化、標準化、產業化生產提供依據。結果錶明,種子萌髮與原毬莖誘導最適培養基為MS+6-BA0.1mg/L+NAA0.5mg/L+20%土豆汁;原毬莖繼代增殖與分化最適培養基為1/2MS+6-BA0.5mg/L+NAA0.1mg/L+20%土豆汁;叢生芽增殖最適培養基為1/2MS+6-BA0.5mg/L+NAA0.5mg/L+20%土豆汁;壯苗生根最適培養基為1/2MS+NAA0.5mg/L+20%土豆汁;煉苗最適溫度、光照及時間:溫度(24±2)℃,光照6000Lx,煉苗時間10d,後3d將瓶蓋打開;試管苗移栽最適基質配方:草碳根+碎木屑+碎石(1:1:1)。
이야생곽산석곡충자위외식체,대곽산석곡조배쾌번기술진행연구,지재위곽산석곡규모화、표준화、산업화생산제공의거。결과표명,충자맹발여원구경유도최괄배양기위MS+6-BA0.1mg/L+NAA0.5mg/L+20%토두즙;원구경계대증식여분화최괄배양기위1/2MS+6-BA0.5mg/L+NAA0.1mg/L+20%토두즙;총생아증식최괄배양기위1/2MS+6-BA0.5mg/L+NAA0.5mg/L+20%토두즙;장묘생근최괄배양기위1/2MS+NAA0.5mg/L+20%토두즙;련묘최괄온도、광조급시간:온도(24±2)℃,광조6000Lx,련묘시간10d,후3d장병개타개;시관묘이재최괄기질배방:초탄근+쇄목설+쇄석(1:1:1)。
In order to provide the basis for the scale, and standardization, industrialization production of Den- drobium huoshanense, research was taken on tissue rapid propagation of Dendrobium huoshanense , which uses the wild Dendrobium huoshanense seed as explants. The results showed that the most suitable medium for seed germination and protocorm induction was MS + 6 - BA 0.1 mg / L + NAA0.5 mg / L + 20% potato juice ; the most suitable medium for protocorms continued metabolic reproduction and differentiation was 1/2MS + 6 - BA 0.5 mg/ L + NAA 0.1 mg/L, +20% potato juice;the most suitable medium for buds increase and multiply was 1/2MS + 6 - BA 0.5 mg / L + NAA0.5 mg / L + 20% potato juice ; the most suitable medium for strong seedling seedling rooting was 1/2MS + NAA0.5 mg/L +20% potato juice;the optimum temperature, light and time for acclimatization is at temperature of (24 ±2)℃, light: the last 3 days; the most suitable formula for plantlets transplant (1: 1: 1). 6000Lx, time: 10days and open the cap was grass carbon root + wood chips + during Gravel