CAJ | 학술논문

目的研究陇东绒山羊细管精液冷冻时，通过在葡萄糖一柠檬酸盐稀释液中添加TCM199，观察其对陇东绒山羊细管冷冻精冷冻效果的影响。方法选用4个试验组和对照组进行对比试验，试验组是在稀释液基础上分别添加TCM199标准液，含量为0．5％、1％、3％、5％（V／V）。结果表明：添加19／6的TCM199标准液的试验组（B）效果较好，其冷冻解冻后精子活力高于对照组和其它试验组，但差异不显著（P〉0．05），质膜完整率试验组和对照组相比差异不显著（P〉0．05），在精子穿卵活力试验中（SPA），试验组和对照组相比差异不显著（P〉0．05）。但在细管解冻后不同时间段活力测定试验中，解冻后5h、8h、12h，试验组（B）和对照组活力差异显著（P〈0．05），且随着时间延长呈下降趋势。因此制作陇东绒山羊精液细管冷冻时，添加1％TCM199标准液具有比较好的效果。
목적연구롱동융산양세관정액냉동시，통과재포도당일저몽산염희석액중첨가TCM199，관찰기대롱동융산양세관냉동정냉동효과적영향。방법선용4개시험조화대조조진행대비시험，시험조시재희석액기출상분별첨가TCM199표준액，함량위0．5％、1％、3％、5％（V／V）。결과표명：첨가19／6적TCM199표준액적시험조（B）효과교호，기냉동해동후정자활력고우대조조화기타시험조，단차이불현저（P〉0．05），질막완정솔시험조화대조조상비차이불현저（P〉0．05），재정자천란활력시험중（SPA），시험조화대조조상비차이불현저（P〉0．05）。단재세관해동후불동시간단활력측정시험중，해동후5h、8h、12h，시험조（B）화대조조활력차이현저（P〈0．05），차수착시간연장정하강추세。인차제작롱동융산양정액세관냉동시，첨가1％TCM199표준액구유비교호적효과。
The effect of TCM-199 supplementation on the Longdong cashmere goat frozen--thawed semen was studied through supplementation of TCM-199 supplementation in the glucose--citrate, a goat semen diluents. Four test groups, which were formulated on the varying basis diluents, doses of 0.5%, 1%, 3% and 5% （V/V）TCM-199, respectively , and control group were selected. The results showed that the test group B diluents was the best one among the diluents tested groups and its semen viability , plasma membrane integrity after--thawing and SPA were all higher than other three test groups and control group and there＇s no obvious statistic difference among them （P〉0.05）. However, semen viability of the test group B was significantly higher than control group diluents after-thawing according to different period （P〈0.05）. The results revealed a decreasing tendency along with the time. Therefore, the test group B was adaptive for making plastic straw frozen semen of Longdong cashmere goat.