CAJ | 학술논문

本研究应用具有免疫荧光特性的抗番鸭呼肠孤病毒病单克隆抗体（MDRV237-11）建立了检测番鸭呼肠孤病毒病抗原的间接免疫荧光（Mab—IFA）方法。结果显示人工感染MDRVMW9710株病毒后发病和死亡番鸭组织切片均可检出阳性病灶；且组织匀浆经尿囊腔途径接种12日龄番鸭胚，取死亡胚心制作冰冻切片进行IFA鉴定呈阳性结果。表明该方法特异性好、可用于快速检测番鸭呼肠孤病毒病。
본연구응용구유면역형광특성적항번압호장고병독병단극륭항체（MDRV237-11）건립료검측번압호장고병독병항원적간접면역형광（Mab—IFA）방법。결과현시인공감염MDRVMW9710주병독후발병화사망번압조직절편균가검출양성병조；차조직균장경뇨낭강도경접충12일령번압배，취사망배심제작빙동절편진행IFA감정정양성결과。표명해방법특이성호、가용우쾌속검측번압호장고병독병。
Monoclonal antibody（MDRV 237--11）against Muscovy duck reovirus was used to build up indirect fluorescence assay（IFA） in order to detect Muscovy duck reovirus （MDRV）. The results showed that frozen section of Muscovy duck infec- ted with MDRV MW9710 strain could appear specific bright green fluorescence by IFA, moreover, 12--old--day Muscovy duck embryo inoculated with liver and spleen homogenate were isolated the virus. So, IFA could replace virus isolation to detect Muscovy duck reovirus rapidly.