CAJ | 학술논문

采用高效液相色谱法分析氧氟沙星在乌骨鸡体内各组织中的残留消除规律。荧光检测器的激发波长为280nm，发射波长为450Dill。色谱柱为ttypersilODS—C18，柱温40~C，以磷酸（以三乙胺调pH至2．5）-乙腈（82：18）为流动相，流速1．0mL／min，进样量为20肛L。氧氟沙星在乌骨鸡肌肉、肝脏、。肾脏中的平均回收率分别为86．3％、83．2％、82．5％。检测限为10ng／g。当组织中氧氟沙星浓度在10～2000ug／kg范围时，线性关系良好。利用该方法对氧氟沙星在乌骨鸡体内的残留消除规律进行了研究，结果表明，氧氟沙星在鸟骨鸡体内的残留消除速度极为缓慢。40天休药期后，2日龄使用组可在肌肉、肝脏和肾脏中分别检出氧氟沙星146．8“g／kg、20．6“g／kg和437．4μg／kg；28日龄使用组分别检测出193．5ug／kg、45．0μg／kg和425．5μg／kg。2日龄使用组休药78天时（即停药71天），肌肉和肝脏中还能分别检出氧氟沙星11．2μg／kg和12．0μg／kg。实验表明乌骨鸡使用氧氟沙星仅适用于10日龄以下给药并休药70天以上，或不给予该类药物。
채용고효액상색보법분석양불사성재오골계체내각조직중적잔류소제규률。형광검측기적격발파장위280nm，발사파장위450Dill。색보주위ttypersilODS—C18，주온40~C，이린산（이삼을알조pH지2．5）-을정（82：18）위류동상，류속1．0mL／min，진양량위20항L。양불사성재오골계기육、간장、。신장중적평균회수솔분별위86．3％、83．2％、82．5％。검측한위10ng／g。당조직중양불사성농도재10～2000ug／kg범위시，선성관계량호。이용해방법대양불사성재오골계체내적잔류소제규률진행료연구，결과표명，양불사성재조골계체내적잔류소제속도겁위완만。40천휴약기후，2일령사용조가재기육、간장화신장중분별검출양불사성146．8“g／kg、20．6“g／kg화437．4μg／kg；28일령사용조분별검측출193．5ug／kg、45．0μg／kg화425．5μg／kg。2일령사용조휴약78천시（즉정약71천），기육화간장중환능분별검출양불사성11．2μg／kg화12．0μg／kg。실험표명오골계사용양불사성부괄용우10일령이하급약병휴약70천이상，혹불급여해류약물。
A reversed-phase high performance liquid chromatographic method has been developed for the determination of ofloxacin residues in black-bone silky fowl. After being extracted with phosphate buffer, ofloxacin was analyzed by HPLC （Hypersil BDS-C18） and detected by FLD （hex = 280 nm, hem = 450 nm） . The mobile phase was acetonitrile-phosphoric acid/triethylamine （18 ： 82）. The linear range was10-2 000p g/kg .The average recovery of ofloxacin was 86.3%, 83.2% or 82.5% in muscle, liver or kindney respectively .The minimal detectable concentration of ofloxacin was 10 ng/g. Results of this experiment showed that the depletion of ofloxacin in muscle and kidney was slow. After 40 days of withdrawal, the concentration of ofloxacin in the chickens used at 2 days old were 146.8μg/kg , 20.6μg/kg or 437.4μg/kg in muscle, liver and kidney respectively, The concentration of ofloxacin in the chickens used at 28 days old were 193.5μg/kg, 45.0μg/kg or 425.5μg/kg respectively. After 71 days of withdrawal, the concentration of ofloxacin in the chickens used at 2 days old were 11.2μg/kg or 12μg/kg in the muscle and liver respectively. This experiment showed that Ofloxacin should only be applied to the chickens younger than 10 days old with more than 70 days of withdrawal or should not be used in raising.