园艺与种苗
園藝與種苗
완예여충묘
Rain Fed Crops
2012年
6期
69-73,86
,共6页
竦椒%细胞核雄性不育%cDNA—AFI-P
竦椒%細胞覈雄性不育%cDNA—AFI-P
송초%세포핵웅성불육%cDNA—AFI-P
chili pepper%Genic male sterility%cDNA-AFLP
[目的]为进一步了解辣椒不育性的分子遗传机理和雄配子的发育机制，对辣椒细胞核雄性不育的育性相关基因进行初步研究。[方法]利用eDNA—AFLP对辣椒细胞核雄性不育两用系‘AB23’的可育株花蕾和不育株花蕾的表达基因进行比较和分析。[结果]可育株与不育株在基因表达上存在差异，筛选出10对引物，并得到了有差异且重复性好且的19条TDFs，其中14条TDFs功能已知，3个功能未知，2个未找到同源序列。该研究通过RT—PCR技术对获得的19个TDFs进行验证，其表达特征与eDNA—AFLP结果一致。[结论]差异基因功能涉及细胞分泌、代谢、转录、细胞程序性死亡、蛋白质修饰、胞间运输、信号转导、细胞黏附等多个相关过程。
[目的]為進一步瞭解辣椒不育性的分子遺傳機理和雄配子的髮育機製，對辣椒細胞覈雄性不育的育性相關基因進行初步研究。[方法]利用eDNA—AFLP對辣椒細胞覈雄性不育兩用繫‘AB23’的可育株花蕾和不育株花蕾的錶達基因進行比較和分析。[結果]可育株與不育株在基因錶達上存在差異，篩選齣10對引物，併得到瞭有差異且重複性好且的19條TDFs，其中14條TDFs功能已知，3箇功能未知，2箇未找到同源序列。該研究通過RT—PCR技術對穫得的19箇TDFs進行驗證，其錶達特徵與eDNA—AFLP結果一緻。[結論]差異基因功能涉及細胞分泌、代謝、轉錄、細胞程序性死亡、蛋白質脩飾、胞間運輸、信號轉導、細胞黏附等多箇相關過程。
[목적]위진일보료해랄초불육성적분자유전궤리화웅배자적발육궤제，대랄초세포핵웅성불육적육성상관기인진행초보연구。[방법]이용eDNA—AFLP대랄초세포핵웅성불육량용계‘AB23’적가육주화뢰화불육주화뢰적표체기인진행비교화분석。[결과]가육주여불육주재기인표체상존재차이，사선출10대인물，병득도료유차이차중복성호차적19조TDFs，기중14조TDFs공능이지，3개공능미지，2개미조도동원서렬。해연구통과RT—PCR기술대획득적19개TDFs진행험증，기표체특정여eDNA—AFLP결과일치。[결론]차이기인공능섭급세포분비、대사、전록、세포정서성사망、단백질수식、포간운수、신호전도、세포점부등다개상관과정。
[Objective] In order to further understand tile molecular genetic mechanism and male gamete development mechanism, the chili nucleic male sterility of sterility genes was studied. [Method] The gene expression of fertile plants buds and sterile plants buds in chili pepper of double line ‘AB23＇were compared and analyzed. [Result] 10 primers were selected and 19 TDFs bands were obtained. Among tbem, 14 were to functionally known genes, 3 function unknown and 2 unmatched. Results of RT-PCR wwere consistent with cDNA-AFLP. [Conclusion] From the analysis of 14 higher homology sequences we could conclude that the 14 homologous genes participated in secretion, metabolism, transcription, programmed cell death, protein modification, cellular transportation, signal transduction, bunding mechanism.
