CAJ | 학술논문

目的：研究香菇多糖对平阳霉素抑制血管内皮瘤细胞的增殖的影响。方法将EOMA小鼠血管内皮瘤细胞分别单用香菇多糖处理（LTN组）、单用平阳霉素处理（BLE组）、香菇多糖联合平阳霉素处理（L+B组）和未做任何处理（CON组），比较4组细胞处理前后细胞增殖和凋亡的差异。结果 CON组和LTN组作用后d 1-d 7的吸光度无显著差异，但均显著高于BLE组和L+B组（P<0.05）；L+B组作用后d 1-d7的吸光度显著低于BLE组（P<0.05）。BLE组和L+B组G 0/G1期和凋亡率显著高于CON组和LTN组（P<0.05），S期、G 2/M期和PI显著低于CON组和LTN组（P<0.05）；L+B组G 0/G1期和凋亡率显著高于BLE组（P<0.05），S期、G 2/M期和PI显著低于BLE组（P<0.05）。结论香菇多糖可显著增强平阳霉素对小鼠血管内皮瘤细胞抑制增殖及促进凋亡的作用。
목적：연구향고다당대평양매소억제혈관내피류세포적증식적영향。방법장EOMA소서혈관내피류세포분별단용향고다당처리（LTN조）、단용평양매소처리（BLE조）、향고다당연합평양매소처리（L+B조）화미주임하처리（CON조），비교4조세포처리전후세포증식화조망적차이。결과 CON조화LTN조작용후d 1-d 7적흡광도무현저차이，단균현저고우BLE조화L+B조（P<0.05）；L+B조작용후d 1-d7적흡광도현저저우BLE조（P<0.05）。BLE조화L+B조G 0/G1기화조망솔현저고우CON조화LTN조（P<0.05），S기、G 2/M기화PI현저저우CON조화LTN조（P<0.05）；L+B조G 0/G1기화조망솔현저고우BLE조（P<0.05），S기、G 2/M기화PI현저저우BLE조（P<0.05）。결론향고다당가현저증강평양매소대소서혈관내피류세포억제증식급촉진조망적작용。
Objective To study the effects of Lentinan on bleomycin A5’s inhibition effects in hemangioma-derived endothelial cell. Methods Hemangioma-derived endothelial cell line EOMA were divided into LTN group (only lentinan treatment), BLE group (only bleomycin A 5 treatment), L+B group (Lentinan and bleomycin A5 treatment) and CON group (no lentinan and bleomycin A5 treatment). Cell proliferation, cell cycle, PI and apoptosis were detected and compared among four groups. Results The differences of absorbance in LBE group was significantly higher than that in L+B group after treatment for d 1-d 7 (P<0.05), but both of them were significantly lower than in CON group and LTN group (P<0.05). The G 0/G 1 stage and apoptosis rate in L+B group was significantly higher than in BLE group(P<0.05), while it was significantly lower in S stage, G 2/M stage and PI(P<0.05). Conclusion Lentinan could significantly promote bleomycin A5’s inhibition effects on hemangioma-derived endothelial cell.