CAJ | 학술논문

本研究选取沈阳地区饲养管理正规的牛规模场，采用液相阻断ELISA试验方法测定牛血清样品中0型-亚洲I型口蹄疫抗体水平，并计算免疫抗体的群体合格率。用0型-亚洲I型二价苗一免、二免、三免的牛在免后28d时牛0型-亚洲I型口蹄疫免疫抗体群体合格率研究结果表明，0型口蹄疫免疫抗体群体合格率分别为82．5％、100％、100％；亚洲I型口蹄疫免疫抗体群体合格率分别为57．5％、97．5％、97．5％。一免后0型和亚洲I型口蹄疫免疫抗体群体合格率下降速度均较快，一免后60d0型免疫抗体群体合格率降到72．5％，免后80d时0型、亚洲I型口蹄疫免疫抗体群体合格率分别降为47．5％、12．5％。二免以上的牛口蹄免疫抗体群体合格率在28～120d内均较一免组高，而且抗体持续时间也长，三免后120d时0型、亚洲I型口蹄疫免疫抗体群体合格率仍高达77．5％、75％。
본연구선취침양지구사양관리정규적우규모장，채용액상조단ELISA시험방법측정우혈청양품중0형-아주I형구제역항체수평，병계산면역항체적군체합격솔。용0형-아주I형이개묘일면、이면、삼면적우재면후28d시우0형-아주I형구제역면역항체군체합격솔연구결과표명，0형구제역면역항체군체합격솔분별위82．5％、100％、100％；아주I형구제역면역항체군체합격솔분별위57．5％、97．5％、97．5％。일면후0형화아주I형구제역면역항체군체합격솔하강속도균교쾌，일면후60d0형면역항체군체합격솔강도72．5％，면후80d시0형、아주I형구제역면역항체군체합격솔분별강위47．5％、12．5％。이면이상적우구제면역항체군체합격솔재28～120d내균교일면조고，이차항체지속시간야장，삼면후120d시0형、아주I형구제역면역항체군체합격솔잉고체77．5％、75％。
In this study, large amount breading field of cattle were chosen to investigate the deamplification rule of type O and type O-Asia I. Liquid phase block ELISA was used to determine the type O-Asia maternal antibody in Cattle and to calculate the group qualified rate.The group qualified rate at day 28 after first, second and third immunization with FMD type O and type O-Asia I combined vaccine are 82.5%, 97.5% and 97.5%.The group qualified rate droped sharply after the first immunization. The type O antibody group qualified rate droped to 72.5% after first immunization at day 60. The type O and type O-Asia I droped to 47.5% and 12.5% after first immunization at day 80. Cattle which injceted the vaccine twice presented a higher group qualified rate than the group which just injected once during day 28 to 120, and the antibody lasted longer. After the third immunization the type O and type O-Asia I group qualified rate remain 77.5% and 75% on day 120.