CAJ | 학술논문

[目的]分析不同温度对水稻胚乳中AGPase各同工酶基因表达水平的影响。[方法]以特青和泰国香米为材料,利用人工气候箱设置高温（日平均温度33℃）和适温（日平均温度25℃）2个温度处理,结合实时荧光定量PCR技术分析比较了水稻淀粉合成关键酶腺嘌呤-葡萄糖焦磷酸化酶（AGPase）7个同工酶基因AGPS1、AGPS2a、AGPS2b、AGPL1、AGPL2、AGPL3及AGPL4的表达特征。[结果]AGPase 3个同工酶基因AGPS2b、AGPL2和AGPL3表达较强,其中AGPL2相对表达量最高;AGPS2b、AGPL2和AGPL3在2个品种中的相对表达量在适温条件下的均高于高温处理,在特青胚乳中各时期2种温度处理下的相对表达量总体高于泰国香米中的相对表达量。[结论]本研究为进一步利用分子生物学技术培育稳定的优质水稻品种提供理论基础。
[목적]분석불동온도대수도배유중AGPase각동공매기인표체수평적영향。[방법]이특청화태국향미위재료,이용인공기후상설치고온（일평균온도33℃）화괄온（일평균온도25℃）2개온도처리,결합실시형광정량PCR기술분석비교료수도정분합성관건매선표령-포도당초린산화매（AGPase）7개동공매기인AGPS1、AGPS2a、AGPS2b、AGPL1、AGPL2、AGPL3급AGPL4적표체특정。[결과]AGPase 3개동공매기인AGPS2b、AGPL2화AGPL3표체교강,기중AGPL2상대표체량최고;AGPS2b、AGPL2화AGPL3재2개품충중적상대표체량재괄온조건하적균고우고온처리,재특청배유중각시기2충온도처리하적상대표체량총체고우태국향미중적상대표체량。[결론]본연구위진일보이용분자생물학기술배육은정적우질수도품충제공이론기출。
[Objective] This study aimed to analyze the effects of temperature on the expression of AGPase isoform genes in rice endosperm during milk stage. [Method] Different temperature treatments （33 and 25 ℃ of daily mean temperature for high and normal temperature treatments, respectively） and the real-time fluorescence quantitative PCR （ FQPCR） were used to analyze the expression patterns of seven isoforms （AGPS1, AGPS2a, AGPS2b, AGPL1, AGPL2, AGPL3 and AGPL4） of ADPglucose pyrophosphorylase （AGPase） which was the key enzyme in starch synthesis and metabolism in rice endosperm of two rice varieties Teqing and Thai Fragrant Rice. [Result] The AGPase isoforms AGPS2b, AGPL2 and AGPL3 had much higher expression than the other four isoforms, thus they were thought to be the main expression patterns of AGPase in rice endosperm. The relative expressions of AGPL2 was the highest among all the isoforms. The relative expressions of AGPS2b, AGPL2 and AGPL3 were higher in the normal temperature treatment than in the high temperature treatment in both rice varieties. The relative expression of the three enzyme genes in milk stages in Teqing was higher than those in Thai Fragrant Rice under different temperature treatments. [Conclusion] This study provides a theoretical basis for further use of molecular biology techniques to cultivate stable high-quality rice varieties.