CAJ | 학술논문

[目的]比较分析普通百里香（Thymus vulgaris L.）组培苗、种子无菌苗和实生苗中精油的化学成分及相对含量,并对精油进行抑菌试验。[方法]利用水蒸气蒸馏法提取精油,采用气质联用法并结合色谱峰面积归一化法分析比较其化学成分和相对百分含量,并用滤纸片法测定其抑菌活性。[结果]3 种样品精油共分离出 54 种化学成分,其主要化学成分相同,均含有百里香酚、对伞花烃、松油烯等,只是相对含量略有差异;抑菌试验中,3 种样品精油对大肠杆菌和金黄色葡萄球菌都有明显的抑菌作用,其中对金黄色葡萄球菌的抑菌作用强于大肠杆菌。[结论]采用离体培养方式生产百里香精油是可行的,其精油的主要化学成分以及抑菌活性基本保持不变。
[목적]비교분석보통백리향（Thymus vulgaris L.）조배묘、충자무균묘화실생묘중정유적화학성분급상대함량,병대정유진행억균시험。[방법]이용수증기증류법제취정유,채용기질련용법병결합색보봉면적귀일화법분석비교기화학성분화상대백분함량,병용려지편법측정기억균활성。[결과]3 충양품정유공분리출 54 충화학성분,기주요화학성분상동,균함유백리향분、대산화경、송유희등,지시상대함량략유차이;억균시험중,3 충양품정유대대장간균화금황색포도구균도유명현적억균작용,기중대금황색포도구균적억균작용강우대장간균。[결론]채용리체배양방식생산백리향정유시가행적,기정유적주요화학성분이급억균활성기본보지불변。
[Objective] This study aimed to compare and analyze the chemical compositions, the relative contents and the antibacterial activity of the essential oils extracted from the tissue culture seedlings, the aseptic seedlings and the common seedlings from seeds of Thymus vulgaris. [Method] The essential oil was extracted by steam distillation; the chemical compositions and their relative contents were analyzed by gas chromatography-mass spectrometry （GC/MS） and peak area normalization method; and the antimicrobial activity was tested by filter paper method. [Result] A total of 54 compositions were identified from these three samples, they had similar main compositions： thymol, m-Cymene, terpinene and so on, and these compositions were slightly different in contents. In addition, the essential oils from these three samples exhibited obvious antibacterial effect against Escherichia coli and Staphylococcus aureus, in which the antibacterial activity against S. aureus was stronger than against E. coli. [Conclusion] It is possible to produce essential oil by tissue culture technology; the main compositions and antibacterial activity of the oils remained almost the same.