CAJ | 학술논문

[目的]利用cpSSR（叶绿体微卫星）分子标记法对丹参的遗传特征进行分析。[方法]选择12对cpSSR扩增良好、重复性好、条带清晰的SSR引物,对全国8省25县31个采样点的丹参进行cpSSR检测分析。[结果]丹参在细胞质遗传（cpSSR）上,总体均体现为中等水平;在地区上存在不同程度差异。基于Shannon多样性指数和Nei’s基因多样性指数的细胞质遗传多样性各省间大小依次为：山西、河南、山东、河北、四川、江苏、陕西、安徽。8省区间丹参的遗传变异以种群间的遗传变异为主导;省区内种群间的基因流有限,而省区间的基因交流较明显。[结论]综合分析认为道地产区和传统主产区的丹参主要为就地引种栽培,在栽培过程中引入了部分外来种源;在道地产区四川、山东、河南之间很早就存在种质互换,新产区多从道地产区引种。但在遗传分化方面未显示地理相关性,进一步说明全国丹参种质之间存在广泛的基因交流,是由于人类活动中对丹参的异地引种所造成。
[목적]이용cpSSR（협록체미위성）분자표기법대단삼적유전특정진행분석。[방법]선택12대cpSSR확증량호、중복성호、조대청석적SSR인물,대전국8성25현31개채양점적단삼진행cpSSR검측분석。[결과]단삼재세포질유전（cpSSR）상,총체균체현위중등수평;재지구상존재불동정도차이。기우Shannon다양성지수화Nei’s기인다양성지수적세포질유전다양성각성간대소의차위：산서、하남、산동、하북、사천、강소、협서、안휘。8성구간단삼적유전변이이충군간적유전변이위주도;성구내충군간적기인류유한,이성구간적기인교류교명현。[결론]종합분석인위도지산구화전통주산구적단삼주요위취지인충재배,재재배과정중인입료부분외래충원;재도지산구사천、산동、하남지간흔조취존재충질호환,신산구다종도지산구인충。단재유전분화방면미현시지리상관성,진일보설명전국단삼충질지간존재엄범적기인교류,시유우인류활동중대단삼적이지인충소조성。
[Objective] This study aimed to analyze the genetic characteristics of Salvia miltiorrhiza by using cpSSR （chloroplast microsatellites） molecular marker method. [Method] Twelve pairs of SSR primers with ideal cpSSR amplification result, good repeatability and clear amplification bands were selected for cpSSR detection and analysis of S. miltiorrhiza samples from 31 sampling locations in 25 counties of 8 provinces in China. [Result] S. miltiorrhiza shows an overall medium level in the cytoplasmic inheritance （cpSSR） and varying degrees of differences in the regions. Based on Shannon’s Information index （I） and Nei’s genetic diversity index, the cytoplasmic genetic diversity in different provinces shows a decreasing order of Shanxi, Henan, Shandong, Hebei, Sichuan, Jiangsu, Shaanxi, Anhui. Genetic variations of S. miltiorrhiza in eight provinces were found mainly among populations. Gene flow among populations with in a province is less than that among different provinces. [Conclusion] Comprehensive analysis shows that S. miltiorrhiza cultivated in genuine producing areas and traditional main producing areas are mainly introduced from the local before introduction of some foreign germplasm resources during cultivation. The exchange of S. miltiorrhiza germplasm resources exists very early among genuine producing areas such as Sichuan, Shandong and Henan, while S. miltiorrhiza germplasm resources in new producing areas are mainly introduced from genuine producing areas. However, no geographical correlation was observed in the genetic differentiation, which further reveals that there is a wide range of genetic exchange among S. miltiorrhiza germplasm resources in China, resulting from the artificial introduction from allopatry.