CAJ | 학술논문

反向遗传操作技术可以在DNA水平上对RNA病毒基因组进行各种修饰或改造,然后通过拯救病毒的表型变化来判断这些基因操作的效果,从而可以对病毒基因组的表达调控机制、病毒致病的分子机理等进行研究。本文就反向遗传操作技术在PRRSV基因功能结构研究以及新型疫苗开发等研究中的应用进行了综述。
반향유전조작기술가이재DNA수평상대RNA병독기인조진행각충수식혹개조,연후통과증구병독적표형변화래판단저사기인조작적효과,종이가이대병독기인조적표체조공궤제、병독치병적분자궤리등진행연구。본문취반향유전조작기술재PRRSV기인공능결구연구이급신형역묘개발등연구중적응용진행료종술。
Reverse genetics can run a variety of modifying and transforming at DNA level for RNA virus genome, and then these genetic manipulation effect is judged by phenotype change of rescued virus. So it can be used to research the expression and regulation mechanism of virus genome and molecular mechanism of virus pathogenicity. This review mainly focused on the progress of applications of reverse genetics in the genome functional analysis and new type of vaccine development of PRRSV.