黑龙江农业科学
黑龍江農業科學
흑룡강농업과학
HEILONGJINAG AGRICULTURAL SCIENCE
2012年
7期
6-13
,共8页
李伟忠%许崇香%安英辉%孙梅%闵丽%姜森%胡国华
李偉忠%許崇香%安英輝%孫梅%閔麗%薑森%鬍國華
리위충%허숭향%안영휘%손매%민려%강삼%호국화
玉米自交系%SSR标记%聚类分析%杂种优势群
玉米自交繫%SSR標記%聚類分析%雜種優勢群
옥미자교계%SSR표기%취류분석%잡충우세군
maize inbred lines%SSR marker%cluster analysis%heterotic groups
为简化杂种优势类群划分,加快育种进程,利用64对SSR核心引物对257份玉米自交系进行遗传多样性分析。结果表明:共检测出422个等位基因变异,每对引物检测出3~9个等位基因,平均6.59个。每个位点的多态性信息量(PIC)为0.444~0.879,平均0.769。257份自交系之间的遗传相似系数变化范围0.036~0.943。按UPGMA类平均方法进行聚类分析,供试自交系可划分为4大类群,6个亚群(Lancaster、旅大红骨、塘四平头、BSSS、PA和PB)。聚类结果与系谱基本一致。
為簡化雜種優勢類群劃分,加快育種進程,利用64對SSR覈心引物對257份玉米自交繫進行遺傳多樣性分析。結果錶明:共檢測齣422箇等位基因變異,每對引物檢測齣3~9箇等位基因,平均6.59箇。每箇位點的多態性信息量(PIC)為0.444~0.879,平均0.769。257份自交繫之間的遺傳相似繫數變化範圍0.036~0.943。按UPGMA類平均方法進行聚類分析,供試自交繫可劃分為4大類群,6箇亞群(Lancaster、旅大紅骨、塘四平頭、BSSS、PA和PB)。聚類結果與繫譜基本一緻。
위간화잡충우세류군화분,가쾌육충진정,이용64대SSR핵심인물대257빈옥미자교계진행유전다양성분석。결과표명:공검측출422개등위기인변이,매대인물검측출3~9개등위기인,평균6.59개。매개위점적다태성신식량(PIC)위0.444~0.879,평균0.769。257빈자교계지간적유전상사계수변화범위0.036~0.943。안UPGMA류평균방법진행취류분석,공시자교계가화분위4대류군,6개아군(Lancaster、려대홍골、당사평두、BSSS、PA화PB)。취류결과여계보기본일치。
To simplify the heterotic group partition,speeding up the breeding process,64 SSR core primers were used to conduct genetic diversity analysis on 257 maize inbred lines.The results showed that:422 allele variance were detected and the mean allele variance number was 6.59 with arrange from 3 to 9 in each site.The mean polymorphism information content(PIC)value was 0.769 with arrange from 0.444 to 0.879.The genetic similarity coefficient of 257 inbred lines ranged from 0.036 to 0.943.257 inbred lines were classified into 4 distinct clusters(6 subgroups),Lancaster,Lvdahonggu,Tangsipingtou,BSSS,PA and PB by the UPGMA analysis which based on genetic distance.The clusting results basically accorded with genealogical sources.