园艺与种苗
園藝與種苗
완예여충묘
Rain Fed Crops
2012年
7期
52-54
,共3页
白玉%高月%赵倩%马慧%陈丽静%钟呜
白玉%高月%趙倩%馬慧%陳麗靜%鐘嗚
백옥%고월%조천%마혜%진려정%종오
紫茉莉%愈伤组织诱导%继代培养
紫茉莉%愈傷組織誘導%繼代培養
자말리%유상조직유도%계대배양
Mirabilis jalapa L. Callus induction Subculture
[目的]该研究以紫茉莉(Mirabilis jalapaL.)为供试植物,建立愈伤组织诱导及继代培养体系,为后期的细胞悬浮培养奠定基础。[方法]以MS为基础培养基,加入不同浓度的6-BA、2,4-D、NAA、KT,设计L16(44)正交试验。[结果]得出最佳愈伤组织诱导组合为:6-BA 0 mg/L+2,4-D 1 mg/L+NAA 1 mg/L+KT 1.5 mg/L,以附加2,4-D 1 mg/L+KT 0.5 mg/L的继代培养基上生长的愈伤组织适合细胞悬浮培养。[结论]筛选出适合紫茉莉愈伤组织培养的激素条件,可为紫茉莉组培相关研究奠定基础。
[目的]該研究以紫茉莉(Mirabilis jalapaL.)為供試植物,建立愈傷組織誘導及繼代培養體繫,為後期的細胞懸浮培養奠定基礎。[方法]以MS為基礎培養基,加入不同濃度的6-BA、2,4-D、NAA、KT,設計L16(44)正交試驗。[結果]得齣最佳愈傷組織誘導組閤為:6-BA 0 mg/L+2,4-D 1 mg/L+NAA 1 mg/L+KT 1.5 mg/L,以附加2,4-D 1 mg/L+KT 0.5 mg/L的繼代培養基上生長的愈傷組織適閤細胞懸浮培養。[結論]篩選齣適閤紫茉莉愈傷組織培養的激素條件,可為紫茉莉組培相關研究奠定基礎。
[목적]해연구이자말리(Mirabilis jalapaL.)위공시식물,건립유상조직유도급계대배양체계,위후기적세포현부배양전정기출。[방법]이MS위기출배양기,가입불동농도적6-BA、2,4-D、NAA、KT,설계L16(44)정교시험。[결과]득출최가유상조직유도조합위:6-BA 0 mg/L+2,4-D 1 mg/L+NAA 1 mg/L+KT 1.5 mg/L,이부가2,4-D 1 mg/L+KT 0.5 mg/L적계대배양기상생장적유상조직괄합세포현부배양。[결론]사선출괄합자말리유상조직배양적격소조건,가위자말리조배상관연구전정기출。
[Objective] Mimbilis jalapa L. was used as the test plant to establish the system of callus induction and subculture for cell suspension culture.[Method] The experiment was designed L16,(44) orthogonal test by adding different type and coneentration of plant gruwth regulating substances,6-BA. 2.4-D.NAA,KT based on MS culture medium. [Resultl The results showed that the appropriate medium tot callus induetion is: 6-BA 0 mg/L +2,4-D 1 mg/L + NAA 1 mg/L + KT 1.5 mg/L.Based on the conclusion.the callus growing on the subculture medium with 2,4-D 1 lng/L + KT 0.5 mg/L was better for (;ell suspension culture. [Conclusion] The suitable hormone level fur Mi,-at,ilis jalapa L callus induction was screened out.These could provide basis for related researches.