CAJ | 학술논문

Ce（Ⅲ）-硝基磺酚C与无色的蛋白质在pH=3．48的HAc—NaAc缓冲介质中生成可溶性蓝色缔合物，hmax=700nm，比试剂本身的最大吸收波长560nm红移了140nm，8=3．95×10^5L·mol^-1·cm^-1，蛋白质在20-140ug／mL（HSA）范围内遵循比尔定律．探讨了反应的最佳条件及初步反应机理．方法灵敏度高，重现性好，操作简便，基本无干扰．BRU-35存在，灵敏度提高19％．用于血清中蛋白质的测定，和目前临床检验中使用的双缩脲法相比，灵敏度提高了11倍．
Ce（Ⅲ）-초기광분C여무색적단백질재pH=3．48적HAc—NaAc완충개질중생성가용성람색체합물，hmax=700nm，비시제본신적최대흡수파장560nm홍이료140nm，8=3．95×10^5L·mol^-1·cm^-1，단백질재20-140ug／mL（HSA）범위내준순비이정률．탐토료반응적최가조건급초보반응궤리．방법령민도고，중현성호，조작간편，기본무간우．BRU-35존재，령민도제고19％．용우혈청중단백질적측정，화목전림상검험중사용적쌍축뇨법상비，령민도제고료11배．
Ce （Ⅲ ） -Nitrosulfophenol C could combine with protein form blue complex in HAc-NaAc buffer at pH 3.48, which presents a maximum absorption at 700nm with 140 nm of red shift compared to Nitrosulfophenol C itself. Its apparent molar absorbency index and linear range were 3.95 × 105 L · mol-1· cm-1 and 20 - 140 μg/mL. The optimum conditions and the initial reaction mechanism were studied. The method is simple, fast, high sensitivity and good reproducibility. Sensitivity increased 19% with the presence of BRIJ-35. The sensitivity of the method is the Biuret method 11 times. The method has been used for the determination of Human serum protein with satisfactory results.