现代农业科技
現代農業科技
현대농업과기
XIANDAIHUA NONGYE
2012年
8期
318-320
,共3页
鳚亚目%线粒体DNA%ITS-1基因序列%系统发育
鳚亞目%線粒體DNA%ITS-1基因序列%繫統髮育
위아목%선립체DNA%ITS-1기인서렬%계통발육
Blennioidei%mitochondrial DNA(mtDNA)%internal transcribed spacer 1(ITS-1)%molecular phylogeny
通过PCR扩增获得了3种中国黄渤海海域的鳚亚目鱼类的线粒体ITS-1基因,并以条斑马鲛为外群,生成供系统发育分析的序列矩阵,利用MEGA 4.0软件分析序列的碱基组成、差异百分比、转换/颠换值等,应用最大简约法(MP)和邻接法(NJ)构建系统发育树。结果表明:在鳚亚目鱼类的ITS-1片段生成的序列矩阵中发现有碱基的插入缺失现象,共有49 bp变异位点,转换/颠换值为1.0;3种鱼ITS-1长度为375~562 bp,其中方氏云鳚的最长,为532~562 bp,六线鳚的最短,为375 bp;锦鳚科的方氏云鳚和云鳚聚为一支;方氏云鳚和云鳚种间遗传距离只有0.02,亲缘关系最近。
通過PCR擴增穫得瞭3種中國黃渤海海域的鳚亞目魚類的線粒體ITS-1基因,併以條斑馬鮫為外群,生成供繫統髮育分析的序列矩陣,利用MEGA 4.0軟件分析序列的堿基組成、差異百分比、轉換/顛換值等,應用最大簡約法(MP)和鄰接法(NJ)構建繫統髮育樹。結果錶明:在鳚亞目魚類的ITS-1片段生成的序列矩陣中髮現有堿基的插入缺失現象,共有49 bp變異位點,轉換/顛換值為1.0;3種魚ITS-1長度為375~562 bp,其中方氏雲鳚的最長,為532~562 bp,六線鳚的最短,為375 bp;錦鳚科的方氏雲鳚和雲鳚聚為一支;方氏雲鳚和雲鳚種間遺傳距離隻有0.02,親緣關繫最近。
통과PCR확증획득료3충중국황발해해역적위아목어류적선립체ITS-1기인,병이조반마교위외군,생성공계통발육분석적서렬구진,이용MEGA 4.0연건분석서렬적감기조성、차이백분비、전환/전환치등,응용최대간약법(MP)화린접법(NJ)구건계통발육수。결과표명:재위아목어류적ITS-1편단생성적서렬구진중발현유감기적삽입결실현상,공유49 bp변이위점,전환/전환치위1.0;3충어ITS-1장도위375~562 bp,기중방씨운위적최장,위532~562 bp,륙선위적최단,위375 bp;금위과적방씨운위화운위취위일지;방씨운위화운위충간유전거리지유0.02,친연관계최근。
In this study,the internal transcribed spacer 1(ITS-1)partial sequences of 3 species from Blennioidei in China yellow and bohai seas were amplified using PCR technique,Scomberomorus regalis was designed as outgroup.Nucleotide composition frequencies,Kimura-2 parameter genetic distance and transition/transversion ratios were analyzed with MEGA 4.0 software.Molecular phylogenetic trees were constructed by maximum parsimony(MP)and neighborjoining(NJ)methods also with MEGA 4.0.The results were as follows:There were insertions and deletions in the aligned base pairs,49 bp variable sites were found in the analysis matrix.,the average ts/tv ratio was 1.0;The length of ITS1 was from 375 bp to 562 bp,with the Enedrias fangi′s the longest,and the Ernogrammus hexagrammus the shortest.Enedrias nebulosus and Enedrias fangi were placed in a same clade with support of a high bootstrap value.Enedrias fangi and Enedrias nebulosus had a close relationship,as the genetic distance between the two species of ITS-1 was only 0.02.