临床肝胆病杂志
臨床肝膽病雜誌
림상간담병잡지
CHINESE JOURNAL OF CLINICAL HEPATOLOGY
2014年
4期
340-343
,共4页
杨莉%冯爱东%郑浩杰%戴二黑
楊莉%馮愛東%鄭浩傑%戴二黑
양리%풍애동%정호걸%대이흑
肝硬化%软肝化坚颗粒%基质金属蛋白酶%金属蛋白酶1 组织抑制剂%小鼠
肝硬化%軟肝化堅顆粒%基質金屬蛋白酶%金屬蛋白酶1 組織抑製劑%小鼠
간경화%연간화견과립%기질금속단백매%금속단백매1 조직억제제%소서
liver cirrhosis%Ruangan Huajian Particle%matrix metalloproteinases%tissue inhibitor of metalloproteinase-1%mice
目的：观察软肝化坚颗粒对肝纤维化模型C57小鼠血清中基质金属蛋白酶13（MMP-13）和基质金属蛋白酶抑制剂1（TIMP-1）的含量和肝组织中MMP-13和TIMP-1 mRNA表达的影响。方法40只C57小鼠被随机分为4组，每组10只，即正常对照组、模型对照组、模型软肝化坚颗粒组和模型秋水仙碱组。采用四氯化碳腹腔注射制造小鼠肝纤维化模型。分别采用ELISA法和实时荧光定量PCR检测小鼠血清MMPs、TIMP-1含量及肝组织中MMP-13和TIMP-1 mRNA的表达。同一指标的多组间计量资料的比较采用方差分析。结果与正常对照组相比较，模型对照组血清中MMPs的含量明显降低，TIMP-1的含量明显升高，P＝0．003、0．027，模型软肝化坚颗粒组与正常对照组相比差异均无统计学意义，P＝0．364、0．217。与正常对照组相比，模型软肝化坚颗粒组小鼠肝组织中MMP-13 mRNA的表达量显著升高，P＝0．005，TIMP-1 mRNA的表达量差异无统计学意义，P＝0.997；模型对照组小鼠肝组织中TIMP-1 mRNA的表达量明显升高，差异具有统计学意义，P＝0．009。结论软肝化坚颗粒不但可促进MMPs的产生，而且可抑制TIMP-1产生，进而促进ECM的降解，这可能也是软肝化坚颗粒抗肝纤维化的重要机制之一。
目的：觀察軟肝化堅顆粒對肝纖維化模型C57小鼠血清中基質金屬蛋白酶13（MMP-13）和基質金屬蛋白酶抑製劑1（TIMP-1）的含量和肝組織中MMP-13和TIMP-1 mRNA錶達的影響。方法40隻C57小鼠被隨機分為4組，每組10隻，即正常對照組、模型對照組、模型軟肝化堅顆粒組和模型鞦水仙堿組。採用四氯化碳腹腔註射製造小鼠肝纖維化模型。分彆採用ELISA法和實時熒光定量PCR檢測小鼠血清MMPs、TIMP-1含量及肝組織中MMP-13和TIMP-1 mRNA的錶達。同一指標的多組間計量資料的比較採用方差分析。結果與正常對照組相比較，模型對照組血清中MMPs的含量明顯降低，TIMP-1的含量明顯升高，P＝0．003、0．027，模型軟肝化堅顆粒組與正常對照組相比差異均無統計學意義，P＝0．364、0．217。與正常對照組相比，模型軟肝化堅顆粒組小鼠肝組織中MMP-13 mRNA的錶達量顯著升高，P＝0．005，TIMP-1 mRNA的錶達量差異無統計學意義，P＝0.997；模型對照組小鼠肝組織中TIMP-1 mRNA的錶達量明顯升高，差異具有統計學意義，P＝0．009。結論軟肝化堅顆粒不但可促進MMPs的產生，而且可抑製TIMP-1產生，進而促進ECM的降解，這可能也是軟肝化堅顆粒抗肝纖維化的重要機製之一。
목적：관찰연간화견과립대간섬유화모형C57소서혈청중기질금속단백매13（MMP-13）화기질금속단백매억제제1（TIMP-1）적함량화간조직중MMP-13화TIMP-1 mRNA표체적영향。방법40지C57소서피수궤분위4조，매조10지，즉정상대조조、모형대조조、모형연간화견과립조화모형추수선감조。채용사록화탄복강주사제조소서간섬유화모형。분별채용ELISA법화실시형광정량PCR검측소서혈청MMPs、TIMP-1함량급간조직중MMP-13화TIMP-1 mRNA적표체。동일지표적다조간계량자료적비교채용방차분석。결과여정상대조조상비교，모형대조조혈청중MMPs적함량명현강저，TIMP-1적함량명현승고，P＝0．003、0．027，모형연간화견과립조여정상대조조상비차이균무통계학의의，P＝0．364、0．217。여정상대조조상비，모형연간화견과립조소서간조직중MMP-13 mRNA적표체량현저승고，P＝0．005，TIMP-1 mRNA적표체량차이무통계학의의，P＝0.997；모형대조조소서간조직중TIMP-1 mRNA적표체량명현승고，차이구유통계학의의，P＝0．009。결론연간화견과립불단가촉진MMPs적산생，이차가억제TIMP-1산생，진이촉진ECM적강해，저가능야시연간화견과립항간섬유화적중요궤제지일。
Objective To observe the effects of Ruangan Huajian granules on the serum levels of matrix metalloproteinase-13 (MMP-13)and tissue inhibitor of metalloproteinase-1 (TIMP-1)and the mRNA expression of MMP-13 and TIMP-1 in liver tissue among C57 mice with he-patic fibrosis.Methods Forty C57 mice were randomly divided into normal control group (n=10),model control group (n=10),Ruangan Hua-jian granule group (n=10),and colchicine group (n=10).A rat model of hepatic fibrosis was established by intraperitoneal injection of carbon tetrachloride in the model control group,Ruangan Huajian granule group,and colchicine group.The serum levels of MMPs and TIMP-1 and the mRNA expression of MMP-13 and TIMP-1 in liver tissue were measured by enzyme-linked immunosorbent assay and real-time quantitative PCR,respectively.Comparison of continuous data between groups was made by analysis of variance.Results Compared with the normal control group,the model control group had a significantly decreased serum level of MMPs (P=0.003)and a significantly increased serum level of TIMP-1 (P=0.027).There were no significant differences in serum levels of MMPs and TIMP-1 between the normal control group and Ruangan Hua-jian granule group (P=0.364 and 0.217).Compared with the normal control group,the Ruangan Huajian granule group had significantly in-creased mRNA expression of MMP-13 in liver tissue (P=0.005),but the mRNA expression of TIMP-1 showed no significant difference between the two groups (P=0.997).The model control group had significantly increased mRNA expression of TIMP-1 in liver tissue compared with the normal control group (P=0.009).Conclusion Ruangan Huajian granules can not only promote the production of MMPs,but also inhibit the pro-duction of TIMP-1,thus promoting extracellular matrix degradation,which may be one of the action mechanisms of Ruangan Huajian granules in the treatment of hepatic fibrosis.
