洛阳师范学院学报
洛暘師範學院學報
락양사범학원학보
JOURNAL OF LUOYANG TEACHERS' COLLEGE
2012年
8期
46-48
,共3页
碘化油%阳离子乳剂%基因给药%绿色荧光
碘化油%暘離子乳劑%基因給藥%綠色熒光
전화유%양리자유제%기인급약%록색형광
iodized oil%Cationic emulsion%gene delivery%green fluorescence
首先制备出粒径为136．5nm、表面电荷为＋24．3mV的碘化油阳离子乳剂，然后以绿色荧光质粒pEG—FP—c1为模型药物，通过在体外细胞培养来观察碘化油阳离子乳剂作为基因载体对机体细胞转染率的影响．体外细胞培养结果显示，碘化油阳离子乳剂复合物的细胞膜穿过率明显提高，转染率远高于裸质粒．
首先製備齣粒徑為136．5nm、錶麵電荷為＋24．3mV的碘化油暘離子乳劑，然後以綠色熒光質粒pEG—FP—c1為模型藥物，通過在體外細胞培養來觀察碘化油暘離子乳劑作為基因載體對機體細胞轉染率的影響．體外細胞培養結果顯示，碘化油暘離子乳劑複閤物的細胞膜穿過率明顯提高，轉染率遠高于裸質粒．
수선제비출립경위136．5nm、표면전하위＋24．3mV적전화유양리자유제，연후이록색형광질립pEG—FP—c1위모형약물，통과재체외세포배양래관찰전화유양리자유제작위기인재체대궤체세포전염솔적영향．체외세포배양결과현시，전화유양리자유제복합물적세포막천과솔명현제고，전염솔원고우라질립．
Iodized oil cationic emulsion＇ s particle diameter is 136.5nm and surface charge is ＋ 24.3mV. The green-fluorescence plasmid pEGFP-Cl is the model drug, and the genetic carrier which effects transfection efficien- cy of body cell culture in vitro is observed. The experiment results indicate that the cellular membrane permeating percentage rises obviously and its transfection efficiency is higher that that of plasmid.
