中国酿造
中國釀造
중국양조
CHINA BREWING
2012年
12期
52-57
,共6页
温顺华%李锋%黄庶冰%廖威%陈丽梅%黄庶识
溫順華%李鋒%黃庶冰%廖威%陳麗梅%黃庶識
온순화%리봉%황서빙%료위%진려매%황서식
褐藻酸盐裂解酶%条件优化%希瓦氏菌%生物能源
褐藻痠鹽裂解酶%條件優化%希瓦氏菌%生物能源
갈조산염렬해매%조건우화%희와씨균%생물능원
为了获得能够将褐藻酸盐水解成为单糖的褐藻酸盐裂解酶,以便于在褐藻生物质能源生产过程中对褐藻的糖化处理,对筛选分离获得的产褐藻酸盐理解解酶菌株Shewanella haliotis BP-1进行产酶条件的初步优化.初步确定了产酶的条件为温度35℃,初始pH8.0,摇床转速200r/min,接种量为1％ (v/v),装液量为500mL三角瓶装200mL,时间24h.优化后的产酶培养基为1.05％ (w/v)K2HPO4,0.45％ (w/v) KH2PO4,0.5％ (w/v) (NH4) 2SO4,2％ (w/v) NaCl,0.2％ (w/v) MgSO4·7H2O,0.001％ (w/v) FeSO4·7H2O,0.4％ (w/v)海藻酸钠.优化后菌株Shewanella haliotis BP-1在发酵培养24h后酶的比活力最高为0.92U/μg,此时发酵液的酶活性为28.1U/mL,发酵48h后达到最大酶活30.4U/mL.
為瞭穫得能夠將褐藻痠鹽水解成為單糖的褐藻痠鹽裂解酶,以便于在褐藻生物質能源生產過程中對褐藻的糖化處理,對篩選分離穫得的產褐藻痠鹽理解解酶菌株Shewanella haliotis BP-1進行產酶條件的初步優化.初步確定瞭產酶的條件為溫度35℃,初始pH8.0,搖床轉速200r/min,接種量為1％ (v/v),裝液量為500mL三角瓶裝200mL,時間24h.優化後的產酶培養基為1.05％ (w/v)K2HPO4,0.45％ (w/v) KH2PO4,0.5％ (w/v) (NH4) 2SO4,2％ (w/v) NaCl,0.2％ (w/v) MgSO4·7H2O,0.001％ (w/v) FeSO4·7H2O,0.4％ (w/v)海藻痠鈉.優化後菌株Shewanella haliotis BP-1在髮酵培養24h後酶的比活力最高為0.92U/μg,此時髮酵液的酶活性為28.1U/mL,髮酵48h後達到最大酶活30.4U/mL.
위료획득능구장갈조산염수해성위단당적갈조산염렬해매,이편우재갈조생물질능원생산과정중대갈조적당화처리,대사선분리획득적산갈조산염리해해매균주Shewanella haliotis BP-1진행산매조건적초보우화.초보학정료산매적조건위온도35℃,초시pH8.0,요상전속200r/min,접충량위1％ (v/v),장액량위500mL삼각병장200mL,시간24h.우화후적산매배양기위1.05％ (w/v)K2HPO4,0.45％ (w/v) KH2PO4,0.5％ (w/v) (NH4) 2SO4,2％ (w/v) NaCl,0.2％ (w/v) MgSO4·7H2O,0.001％ (w/v) FeSO4·7H2O,0.4％ (w/v)해조산납.우화후균주Shewanella haliotis BP-1재발효배양24h후매적비활력최고위0.92U/μg,차시발효액적매활성위28.1U/mL,발효48h후체도최대매활30.4U/mL.
