中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2013年
6期
660-663
,共4页
孙林春%张兰芳%张利%胡正
孫林春%張蘭芳%張利%鬍正
손림춘%장란방%장리%호정
手足口病%儿童%T淋巴细胞亚群%细胞免疫%流式细胞仪
手足口病%兒童%T淋巴細胞亞群%細胞免疫%流式細胞儀
수족구병%인동%T림파세포아군%세포면역%류식세포의
Hand,foot and mouth disease%Children%T lymphocyte subsets%Cellular immunity%Flow cytometry
目的 观察386例手足口病(HFMD)患儿外周血T淋巴细胞亚群数量在整个病程中的变化,探讨其临床意义.方法 用流式细胞仪对386例手足口病患儿(手足口病组)和78名健康儿童(健康对照组)外周血T淋巴细胞亚群进行检测.386例手足口病患儿按病情轻重程度分,325例为普通型(普通型手足口病组),61例为重症型(重型手足口病组)；按病程的不同阶段分,264例为发病期(发病期手足口病组),122例为恢复期(恢复期手足口病组).统计分析手足口病患儿与健康儿童,普通型与重症型手足口病患儿,恢复期与发病期手足口病患儿外周血T淋巴细胞亚群数量的差异.结果 手足口病组外周血CD3+、CD4+、CD8+细胞相对计数和CD4 +/CD8+比值依次为(47.4±10.6)％、(24.0±8.0)％、(23.2±7.4)％和1.0±0.5,与健康对照组各项指标[(68.4±7.5)％、(38.2±6.9)％、(18.8±4.6)％和2.0±0.4]比较差异均有统计学意义(t值分别为3.16、4.62、2.23、2.51,P均＜0.05)；普通型手足口病组外周血CD3+、CD4+、CD8+细胞相对计数和CD4+/CD8+比值分别为[(53.0±9.8)％、(26.5±7.9)％、(20.4±7.5)％和1.3±0.6,与重型手足口病组各项指标[(42.3±10.9)％、(18.7±4.7)％、(24.2±7.4)％和0.7±0.4]比较差异均有统计学意义(t值分别为2.25、2.47、1.91、2.32,P均＜0.05)；发病期手足口病组外周血CD3+、CD4+细胞相对计数和CD4 +/CD8+比值分别为(45.3±10.5)％、(21.6±8.4)％和0.9±0.5,与恢复期手足口病组[(54.7±9.9)％、(27.5±9.1)％和1.4±0.6]比较差异均有统计学意义(t值分别为2.41、1.87、2.92,P均＜0.05).结论 手足口病患儿的细胞免疫受损,T淋巴细胞功能处于抑制状态,且抑制程度与病情轻重呈正相关.
目的 觀察386例手足口病(HFMD)患兒外週血T淋巴細胞亞群數量在整箇病程中的變化,探討其臨床意義.方法 用流式細胞儀對386例手足口病患兒(手足口病組)和78名健康兒童(健康對照組)外週血T淋巴細胞亞群進行檢測.386例手足口病患兒按病情輕重程度分,325例為普通型(普通型手足口病組),61例為重癥型(重型手足口病組)；按病程的不同階段分,264例為髮病期(髮病期手足口病組),122例為恢複期(恢複期手足口病組).統計分析手足口病患兒與健康兒童,普通型與重癥型手足口病患兒,恢複期與髮病期手足口病患兒外週血T淋巴細胞亞群數量的差異.結果 手足口病組外週血CD3+、CD4+、CD8+細胞相對計數和CD4 +/CD8+比值依次為(47.4±10.6)％、(24.0±8.0)％、(23.2±7.4)％和1.0±0.5,與健康對照組各項指標[(68.4±7.5)％、(38.2±6.9)％、(18.8±4.6)％和2.0±0.4]比較差異均有統計學意義(t值分彆為3.16、4.62、2.23、2.51,P均＜0.05)；普通型手足口病組外週血CD3+、CD4+、CD8+細胞相對計數和CD4+/CD8+比值分彆為[(53.0±9.8)％、(26.5±7.9)％、(20.4±7.5)％和1.3±0.6,與重型手足口病組各項指標[(42.3±10.9)％、(18.7±4.7)％、(24.2±7.4)％和0.7±0.4]比較差異均有統計學意義(t值分彆為2.25、2.47、1.91、2.32,P均＜0.05)；髮病期手足口病組外週血CD3+、CD4+細胞相對計數和CD4 +/CD8+比值分彆為(45.3±10.5)％、(21.6±8.4)％和0.9±0.5,與恢複期手足口病組[(54.7±9.9)％、(27.5±9.1)％和1.4±0.6]比較差異均有統計學意義(t值分彆為2.41、1.87、2.92,P均＜0.05).結論 手足口病患兒的細胞免疫受損,T淋巴細胞功能處于抑製狀態,且抑製程度與病情輕重呈正相關.
목적 관찰386례수족구병(HFMD)환인외주혈T림파세포아군수량재정개병정중적변화,탐토기림상의의.방법 용류식세포의대386례수족구병환인(수족구병조)화78명건강인동(건강대조조)외주혈T림파세포아군진행검측.386례수족구병환인안병정경중정도분,325례위보통형(보통형수족구병조),61례위중증형(중형수족구병조)；안병정적불동계단분,264례위발병기(발병기수족구병조),122례위회복기(회복기수족구병조).통계분석수족구병환인여건강인동,보통형여중증형수족구병환인,회복기여발병기수족구병환인외주혈T림파세포아군수량적차이.결과 수족구병조외주혈CD3+、CD4+、CD8+세포상대계수화CD4 +/CD8+비치의차위(47.4±10.6)％、(24.0±8.0)％、(23.2±7.4)％화1.0±0.5,여건강대조조각항지표[(68.4±7.5)％、(38.2±6.9)％、(18.8±4.6)％화2.0±0.4]비교차이균유통계학의의(t치분별위3.16、4.62、2.23、2.51,P균＜0.05)；보통형수족구병조외주혈CD3+、CD4+、CD8+세포상대계수화CD4+/CD8+비치분별위[(53.0±9.8)％、(26.5±7.9)％、(20.4±7.5)％화1.3±0.6,여중형수족구병조각항지표[(42.3±10.9)％、(18.7±4.7)％、(24.2±7.4)％화0.7±0.4]비교차이균유통계학의의(t치분별위2.25、2.47、1.91、2.32,P균＜0.05)；발병기수족구병조외주혈CD3+、CD4+세포상대계수화CD4 +/CD8+비치분별위(45.3±10.5)％、(21.6±8.4)％화0.9±0.5,여회복기수족구병조[(54.7±9.9)％、(27.5±9.1)％화1.4±0.6]비교차이균유통계학의의(t치분별위2.41、1.87、2.92,P균＜0.05).결론 수족구병환인적세포면역수손,T림파세포공능처우억제상태,차억제정도여병정경중정정상관.
Objective To detect the changes of peripheral blood T lymphocyte subsets in patients with hand,foot and mouth disease (HFMD) and investigate its clinical significance.Methods Three hundred and eighty-six patients with HFMD and 78 healthy children were detected the peripheral blood T lymphocyte subsets levels using the flow cytometry (FCM).The patients were divided into 325 common patients and 61 critically ill patients according to the severity of disease.Among the 386 patients,264 were in infection stage and 122 were in recovery stage.Compared the differences of peripheral blood T lymphocyte subsets levels in patients and healthy controls,common patients and critically ill patients,infection patients and recovery patients respectively.Results The patients' numbers of CD3 +,CD4 +,CD8 + cells and CD4 +/CD8 + ratio were (47.4 ± 10.6) ％,(24.0 ±8.0) ％,(23.2 ± 7.4) ％ and 1.0 ± 0.5 respectively.These parameters were significantly different with those in the control group ((68.4±7.5)％,(38.2 ±6.9)％,(18.8 ±4.6)％ and 2.0 ±0.4 respectively;t =3.16,4.62,2.23 and 2.51 respectively,P ＜ 0.05).The common patients' numbers of CD3 +,CD4 +,CD8 + cells and CD4+/CD8 + ratio were (53.0 ±9.8)％,(26.5 ±7.9)％,(20.4±7.5)％and 1.3 ±0.6,which had significant differences compared with those of the critically ill patients ((42.3 ± 10.9) ％,(18.7 ± 4.7) ％,(24.2 ± 7.4) ％ and (0.7 ± 0.4) respectively; t =2.25,2.47,1.91 and 2.32 respectively,P ＜ 0.05).The infection patients' CD3 +,CD4 +,cell numbers and CD4 +/CD8 + ratio were (45.3 ± 10.5) ％,(21.6 ± 8.4) ％and 0.9 ± 0.5.These parameters were significantly different with those of the recovery patients ((54.7 ±9.9)％,(27.5 ± 9.1)％ and 1.4 ± 0.6 respectively; t =2.41,1.87 and 2.92 respectively,P ＜ 0.05).Conclusion The cellular immunity of patients with HFMD was impaired.The T lymphocyte function was suppressed,and the level of suppression was positively correlated to severity of the illness.
