广州化工
廣州化工
엄주화공
GUANGZHOU CHEMICAL INDUSTRY AND TECHNOLOGY
2012年
6期
99-100,119
,共3页
南丹%蒋丽丹%蔡秋香%朱依娜%王良贵
南丹%蔣麗丹%蔡鞦香%硃依娜%王良貴
남단%장려단%채추향%주의나%왕량귀
30%松脂酸钠水乳剂%生物农药%反相高效液相色谱
30%鬆脂痠鈉水乳劑%生物農藥%反相高效液相色譜
30%송지산납수유제%생물농약%반상고효액상색보
30% sodium abietate aqueous emulsion%biopesticide%RP-HPLC
采用ZORBAX SB-C18柱,检测波长245 nm,以甲醇-pH 3.0磷酸缓冲溶液(体积比70∶30)为流动相,流速0.7 mL/min色谱分析条件。建立了30%松脂酸钠水乳剂生物农药的反相高效液相色谱分析方法。结果表明,松脂酸保留时间14.919 min,松脂酸浓度在2.022~80.88 mg/L时其峰面积与进样浓度呈良好的线性关系,方法回收率90.51%~110.2%,RSD为7.37%,最低检出限0.179 mg/L。该方法灵敏、可靠,具有简便、重现性好的特点。
採用ZORBAX SB-C18柱,檢測波長245 nm,以甲醇-pH 3.0燐痠緩遲溶液(體積比70∶30)為流動相,流速0.7 mL/min色譜分析條件。建立瞭30%鬆脂痠鈉水乳劑生物農藥的反相高效液相色譜分析方法。結果錶明,鬆脂痠保留時間14.919 min,鬆脂痠濃度在2.022~80.88 mg/L時其峰麵積與進樣濃度呈良好的線性關繫,方法迴收率90.51%~110.2%,RSD為7.37%,最低檢齣限0.179 mg/L。該方法靈敏、可靠,具有簡便、重現性好的特點。
채용ZORBAX SB-C18주,검측파장245 nm,이갑순-pH 3.0린산완충용액(체적비70∶30)위류동상,류속0.7 mL/min색보분석조건。건립료30%송지산납수유제생물농약적반상고효액상색보분석방법。결과표명,송지산보류시간14.919 min,송지산농도재2.022~80.88 mg/L시기봉면적여진양농도정량호적선성관계,방법회수솔90.51%~110.2%,RSD위7.37%,최저검출한0.179 mg/L。해방법령민、가고,구유간편、중현성호적특점。
The method for the determination of the sodium abietate content in 30% sodium abietate aqueous emulsion was established by reversed-phase high performance liquid chromatography.The chromatography was carried out on a Agilentl100 with ZORBAX SB-C18 column(250 mm × 4.6 mm i.d.,5 μm),a mobile phase of the methanol-phosphorous acid buffer solution(pH3.0)=70:30(by vol) at a flow rate of 0.7 mL/min and the detection wavelength at 241 nm.The quantitative analysis was made by using external standard method.The results showed that the retention time of abietate acid was at 14.919 min,and a good linearity between the content of abietate acid and the chromatography area in the range from 2.022 to 80.88 mg/L.The relative standard deviation of the method and the detect ion limit was 7.37% and 0.179 mg/L,respectively.The recovery of the method was 90.51%~110.2%.This method was sensitive,simple and accurate.