CAJ | 학술논문

以1,10-菲罗啉、壳聚糖和四水合乙酸钴为原料,合成了水溶性壳聚糖钴（II）配合物（CHT-Co（II））,采用红外光谱对配体及配合物进行了表征。在pH 7.5的Tris-HCl缓冲溶液中,采用粘度法、紫外可见吸收光谱法和电化学方法（循环伏安法,微分脉冲伏安法）研究了配合物与DNA的相互作用。粘度法表明二者能发生部分嵌插作用;DNA的加入能引起配合物紫外光谱的变化也表明了二者之间的相互作用。电化学实验表明配合物在玻碳电极上的氧化还原为一可逆过程,当加入一定量的DNA时,配合物的氧化峰电流明显降低,式电位有所正移,验证了二者之间存在部分嵌插作用。
이1,10-비라람、각취당화사수합을산고위원료,합성료수용성각취당고（II）배합물（CHT-Co（II））,채용홍외광보대배체급배합물진행료표정。재pH 7.5적Tris-HCl완충용액중,채용점도법、자외가견흡수광보법화전화학방법（순배복안법,미분맥충복안법）연구료배합물여DNA적상호작용。점도법표명이자능발생부분감삽작용;DNA적가입능인기배합물자외광보적변화야표명료이자지간적상호작용。전화학실험표명배합물재파탄전겁상적양화환원위일가역과정,당가입일정량적DNA시,배합물적양화봉전류명현강저,식전위유소정이,험증료이자지간존재부분감삽작용。
A cobalt complex with 1,10-phenanthroline and water-soluble chitosan（CHT-Co（Ⅱ）） as ligands was synthesized and characterized.In pH 7.5 Tris-HCl buffer solution,the interaction of the cobalt complex with DNA was investigated by viscosity UV-visible absorption spectroscopy,and electrochemical methods（cyclic voltammetry,differential pulse voltammetry）.Viscosity experiments revealed that the cobalt complex bind to DNA via partial intercalation.UV-spectrum of the CHT-Co（Ⅱ） complex was changed when DNA were added,proving the interaction of the two species.The electrochemistry of the complex on a glassy carbon electrode showed a pair of reversible redox potential corresponding to the electron transfer of Co（Ⅱ）/Co（I） couple.When a certain amount of DNA was added,the peak current of the complex decreasedy,with a positive shift of peak potential,confirming the intercalative mode between the CHT-Co（Ⅱ） complex and DNA.