交通医学
交通醫學
교통의학
MEDICAL JOURNAL OF COMMUNICATIONS
2013年
6期
607-610
,共4页
小细胞肺癌%Nrf2%c-myc癌基因%Western-Blot检测法
小細胞肺癌%Nrf2%c-myc癌基因%Western-Blot檢測法
소세포폐암%Nrf2%c-myc암기인%Western-Blot검측법
small cell lung cancer%Nrf2%c-myc oncogene%Western-Blot assay
目的：探讨小细胞肺癌c-myc癌基因的表达对Nrf2表达的影响，分析c-myc与Nrf2表达的相关性。方法：选用两株耐药性不同的肺小细胞癌肿瘤细胞株，小细胞肺癌药物敏感株H69及耐药株H69AR，Western-Blot方法比较Nrf2，c-myc在两株细胞间的表达差异；H69细胞通过Lipofectamine2000脂质体转染过表达c-myc，Western-Blot方法检测过表达c-myc前后Nrf2蛋白表达变化；H69AR细胞通过Lipofectamine2000脂质体转染c-myc siRNA， Western-Blot方法检测c-myc siRNA前后Nrf2的蛋白表达变化。结果：与H69细胞相比，H69AR细胞株中c-myc及Nrf2高表达；H69细胞c-myc转染后，Nrf2表达上调；H69AR细胞c-myc siRNA干扰后，Nrf2表达下调。结论：小细胞肺癌中Nrf2的表达与c-myc表达呈正相关。
目的：探討小細胞肺癌c-myc癌基因的錶達對Nrf2錶達的影響，分析c-myc與Nrf2錶達的相關性。方法：選用兩株耐藥性不同的肺小細胞癌腫瘤細胞株，小細胞肺癌藥物敏感株H69及耐藥株H69AR，Western-Blot方法比較Nrf2，c-myc在兩株細胞間的錶達差異；H69細胞通過Lipofectamine2000脂質體轉染過錶達c-myc，Western-Blot方法檢測過錶達c-myc前後Nrf2蛋白錶達變化；H69AR細胞通過Lipofectamine2000脂質體轉染c-myc siRNA， Western-Blot方法檢測c-myc siRNA前後Nrf2的蛋白錶達變化。結果：與H69細胞相比，H69AR細胞株中c-myc及Nrf2高錶達；H69細胞c-myc轉染後，Nrf2錶達上調；H69AR細胞c-myc siRNA榦擾後，Nrf2錶達下調。結論：小細胞肺癌中Nrf2的錶達與c-myc錶達呈正相關。
목적：탐토소세포폐암c-myc암기인적표체대Nrf2표체적영향，분석c-myc여Nrf2표체적상관성。방법：선용량주내약성불동적폐소세포암종류세포주，소세포폐암약물민감주H69급내약주H69AR，Western-Blot방법비교Nrf2，c-myc재량주세포간적표체차이；H69세포통과Lipofectamine2000지질체전염과표체c-myc，Western-Blot방법검측과표체c-myc전후Nrf2단백표체변화；H69AR세포통과Lipofectamine2000지질체전염c-myc siRNA， Western-Blot방법검측c-myc siRNA전후Nrf2적단백표체변화。결과：여H69세포상비，H69AR세포주중c-myc급Nrf2고표체；H69세포c-myc전염후，Nrf2표체상조；H69AR세포c-myc siRNA간우후，Nrf2표체하조。결론：소세포폐암중Nrf2적표체여c-myc표체정정상관。
Objective:To determine the influence of oncogene c-myc over Nrf2 in small cell lung cancer, and to an-alyze the correlation between c-myc and Nrf2 expressions. Methods:Two lines of small-cell lung cancer cells were chosen in this study. One is drug sensitive H69 cell. The other is multidrug resistant H69AR cell. Western blot assays were uti-lized to compare the different expression levels of c-myc and Nrf2. Overexpression of c-myc by Lipofectamine 2000 trans-fection was performed in H69 cells, and Nrf2 protein expression was compared between the control and c-myc transfection groups. Down-regulation of c-myc by siRNA transfection was performed in H69AR cells, and Nrf2 protein expression was compared between the control and c-myc siRNA groups. Results: Compared with H69 cells, protein expression level of c-myc and Nrf2 was much higher in H69AR cell. In H69 tumor cells, Nrf2 was up-regulated after c-myc transfection. Mean-while, in H69AR tumor cells, Nrf2 was down-regulated after c-myc siRNA transfection. Conclusion: Nrf2 and c-myc is positively correlated in small cell lung cancer. To determine the influence of c-myc over Nrf2 in small cell lung cancer, and to analyse the correlation between c-myc and Nrf2 expressions.
