CAJ | 학술논문

目的：探讨慢性HBV感染者特异性细胞毒性T淋巴细胞（specific cytotoxic T lymphocyte，CTL）及人类白细胞抗原-A2（human leucocyte antigen-A2，HLA-A2）的变化,并分析CTL与血清丙氨酸氨基转移酶（alanine aminotransferase，ALT）水平和病毒载量的关系，以了解慢性HBV感染者特异性细胞免疫功能的状态。方法：采用HLA-A2限制的HBVcore18-27抗原表位肽五聚体(MHC Pentamers)法结合流式细胞技术，分析CHB组慢性乙型肝炎46例、LC组乙型肝炎肝硬化29例和NC组23例正常对照健康体检者，外周血HBV特异性CTL及HLA-A2的变化，并分析CTL与ALT水平和病毒载量的关系。结果：CHB组、LC组HLA-A2检出率均明显高于NC组；ALT水平和病毒载量与HBV特异性的CTL有一定的关系。结论：采用HLA-A2限制的HBVcore18-27抗原表位肽五聚体(MHC Pentamers)法结合流式细胞技术可以检测到低水平的HBV特异性CTL；HLA-A2是乙型肝炎肝硬化的易感基因之一；ALT水平和病毒载量与HBV特异性CTL有一定的关系。
목적：탐토만성HBV감염자특이성세포독성T림파세포（specific cytotoxic T lymphocyte，CTL）급인류백세포항원-A2（human leucocyte antigen-A2，HLA-A2）적변화,병분석CTL여혈청병안산안기전이매（alanine aminotransferase，ALT）수평화병독재량적관계，이료해만성HBV감염자특이성세포면역공능적상태。방법：채용HLA-A2한제적HBVcore18-27항원표위태오취체(MHC Pentamers)법결합류식세포기술，분석CHB조만성을형간염46례、LC조을형간염간경화29례화NC조23례정상대조건강체검자，외주혈HBV특이성CTL급HLA-A2적변화，병분석CTL여ALT수평화병독재량적관계。결과：CHB조、LC조HLA-A2검출솔균명현고우NC조；ALT수평화병독재량여HBV특이성적CTL유일정적관계。결론：채용HLA-A2한제적HBVcore18-27항원표위태오취체(MHC Pentamers)법결합류식세포기술가이검측도저수평적HBV특이성CTL；HLA-A2시을형간염간경화적역감기인지일；ALT수평화병독재량여HBV특이성CTL유일정적관계。
Objective:To investigate the changes of specific cytotoxic T lymphocytes(CTL)and HLA-A2 in patients with chronic HBV infection and to analyze the relationships between the CTL and ALT and HBV copies, all of which can facilitate us to understand the statue of specific cellular immunity functions in patients with chronic HBV infection. Meth-ods: Peripheral HBV specific CTL and HLA-A2 of 46 patients with chronic hepatitis B, 29 patients with chronic hepatitis B cirrhosis and 13 healthy examined people (as normal controls) were measured by HLA-A2 limited HBV core 18-27 pep-tide pentamers combined with Flow Cytometry. The relationships between the CTL and ALT and HBV copies were anal-ysed. Results:The detection rate of patients with chronic hepatitis B and cirrhosis were higher than that of the normal con-trol group. The level of ALT and the amount of HBV copies were related closely to HBV specific CTL. Conclusion: The low level HBV specific CTL can be detected by the method we adopted, the HLA-A2 was one of the susceptibility genes for hepatitis B cirrhosis, and the level of ALT and the amount of HBV copies were related closely to HBV specific CTL.