交通医学
交通醫學
교통의학
MEDICAL JOURNAL OF COMMUNICATIONS
2013年
6期
585-588
,共4页
王晓珂%赵健亚%刘天娥%张璇%吴俊霞%王春%陈刚
王曉珂%趙健亞%劉天娥%張璇%吳俊霞%王春%陳剛
왕효가%조건아%류천아%장선%오준하%왕춘%진강
肥胖易感%肥胖抵抗%体重%水甘油通道蛋白7%荧光实时定量聚合酶链反应%大鼠
肥胖易感%肥胖牴抗%體重%水甘油通道蛋白7%熒光實時定量聚閤酶鏈反應%大鼠
비반역감%비반저항%체중%수감유통도단백7%형광실시정량취합매련반응%대서
Obesity prone%Obesity resistance%Body weight%aquaporins 7%real time polymerase chain reaction
目的：探讨水甘油通道蛋白7与高脂膳食诱导的大鼠肥胖的关系。方法：雄性6周龄SD大鼠随机分为高脂饲料组24只，普通饲料组10只。8周后将高脂饲料组大鼠分为饮食诱导肥胖组(OP)和饮食诱导肥胖抵抗组(OR)大鼠，口服葡萄糖耐量实验检测各组大鼠的胰岛素敏感性。实验结束分离大鼠的肾周和睾周脂肪组织并准确称重，分离血清测定血糖血脂。实时荧光定量PCR技术检测大鼠脂肪组织AQP7的mRNA表达。结果：OP组大鼠体重（556.8±10.6）g、体脂（6.47±0.48）%、TC(1.43±0.14）mmoL/L、TG（0.78±0.13）mmoL/L、Insulin含量（66.09±8.06）μIU/mL和HOMA-IR （19.63±3.08）。显著高于OR组的（478.9±8.9）g、（4.53±0.43）%、（1.15±0.10）mmoL/L、（0.62±0.04）mmoL/L、（48.03±8.70）μIU/mL、（14.74±9.61）和对照组（470.5±8.6）g、（3.34±0.28）%、（1.17±0.15）mmoL/L、（0.51±0.08）mmoL/L、（29.57±6.94）μIU/mL、（8.08±0.81）。OP组AQP7的mRNA表达（3.80±0.38）与OR组（1.12±0.15）和对照组（1.00±0.00）相比也显著增加，OR组和C组相比差异无统计学意义。结论：脂肪组织中AQP7表达与高脂膳食诱导的肥胖密切相关。
目的：探討水甘油通道蛋白7與高脂膳食誘導的大鼠肥胖的關繫。方法：雄性6週齡SD大鼠隨機分為高脂飼料組24隻，普通飼料組10隻。8週後將高脂飼料組大鼠分為飲食誘導肥胖組(OP)和飲食誘導肥胖牴抗組(OR)大鼠，口服葡萄糖耐量實驗檢測各組大鼠的胰島素敏感性。實驗結束分離大鼠的腎週和睪週脂肪組織併準確稱重，分離血清測定血糖血脂。實時熒光定量PCR技術檢測大鼠脂肪組織AQP7的mRNA錶達。結果：OP組大鼠體重（556.8±10.6）g、體脂（6.47±0.48）%、TC(1.43±0.14）mmoL/L、TG（0.78±0.13）mmoL/L、Insulin含量（66.09±8.06）μIU/mL和HOMA-IR （19.63±3.08）。顯著高于OR組的（478.9±8.9）g、（4.53±0.43）%、（1.15±0.10）mmoL/L、（0.62±0.04）mmoL/L、（48.03±8.70）μIU/mL、（14.74±9.61）和對照組（470.5±8.6）g、（3.34±0.28）%、（1.17±0.15）mmoL/L、（0.51±0.08）mmoL/L、（29.57±6.94）μIU/mL、（8.08±0.81）。OP組AQP7的mRNA錶達（3.80±0.38）與OR組（1.12±0.15）和對照組（1.00±0.00）相比也顯著增加，OR組和C組相比差異無統計學意義。結論：脂肪組織中AQP7錶達與高脂膳食誘導的肥胖密切相關。
목적：탐토수감유통도단백7여고지선식유도적대서비반적관계。방법：웅성6주령SD대서수궤분위고지사료조24지，보통사료조10지。8주후장고지사료조대서분위음식유도비반조(OP)화음식유도비반저항조(OR)대서，구복포도당내량실험검측각조대서적이도소민감성。실험결속분리대서적신주화고주지방조직병준학칭중，분리혈청측정혈당혈지。실시형광정량PCR기술검측대서지방조직AQP7적mRNA표체。결과：OP조대서체중（556.8±10.6）g、체지（6.47±0.48）%、TC(1.43±0.14）mmoL/L、TG（0.78±0.13）mmoL/L、Insulin함량（66.09±8.06）μIU/mL화HOMA-IR （19.63±3.08）。현저고우OR조적（478.9±8.9）g、（4.53±0.43）%、（1.15±0.10）mmoL/L、（0.62±0.04）mmoL/L、（48.03±8.70）μIU/mL、（14.74±9.61）화대조조（470.5±8.6）g、（3.34±0.28）%、（1.17±0.15）mmoL/L、（0.51±0.08）mmoL/L、（29.57±6.94）μIU/mL、（8.08±0.81）。OP조AQP7적mRNA표체（3.80±0.38）여OR조（1.12±0.15）화대조조（1.00±0.00）상비야현저증가，OR조화C조상비차이무통계학의의。결론：지방조직중AQP7표체여고지선식유도적비반밀절상관。
Objective:To establish high fat diet induced obesity prone and obesity resistant rats and investigate the AQP7 expression in white adipose tissue in each group. Methods: six-week-old outbred male SD rats were randomly di-vided into two groups: the high-fat diet group (HF, n=24) and the chow food group (C, n=10). After 8 weeks, rats in HF group were designated as OP and OR rats. Insulin sensitivity was determined by oral glucose tolerance test. AQP7 mRNA expression was detected by Real time Polymerase Chain Reaction (RT-PCR). Results:Among OP , OR group and C group, body weights were (556.8±10.6), (478.9±8.9) and (470.5±8.6)g, (P<0.05 );body fat was (6.47±0.48), (4.53±0.43) and (3.34± 0.28)%, (P<0.05);Serum TC was (1.43±0.14), (1.15±0.10) and (1.17±0.15), (P<0.05);TG was (0.78±0.13), (0.62±0.04) and (0.51 ±0.08), (P<0.05); Insulin was (66.09 ±8.06), (48.03 ±8.70) and (29.57 ±6.94), (P<0.05); HOMA-IR was (19.63 ±3.08), (14.74±9.51) and (8.08±0.81), (P<0.05); AQP7 mRNA expression in white adipose tissue was (3.80±0.38), (1.12±0.15) and (1.00±0.00), (P<0.05). Conclusion: AQP7 mRNA expression in white adipose tissue is closely related to high-fat diet in-duced obesity.
