CAJ | 학술논문

以Bacillus DE-8为试验菌株,选用不同的油脂下脚料为发酵基质,对其降解条件进行正交优化研究.结果表明：BacillusDE-8发酵的最佳培养条件是起始 pH 值为8,接种量4%、摇床转速为160 r/min、温度为32℃、添加1%氯化钙及1%表面活性剂十二苯磺酸钠,发酵72 h,在该条件下,验证试验残油降解率达81.6%.
이Bacillus DE-8위시험균주,선용불동적유지하각료위발효기질,대기강해조건진행정교우화연구.결과표명：BacillusDE-8발효적최가배양조건시기시 pH 치위8,접충량4%、요상전속위160 r/min、온도위32℃、첨가1%록화개급1%표면활성제십이분광산납,발효72 h,재해조건하,험증시험잔유강해솔체81.6%.
Taking Bacillus DE-8 as the original strain,choosing different oil wastes as fermentation me-dium,the degradation conditions were orthogonal optimized.The results showed that the optimal fermenta-tion conditions were as follows,initial pH 8.0,inoculability quantity 4%,rotate speed 160 r/min,tempera-ture 32 ℃,CaCl2 1% and NaDDBS 1%.Under these conditions,The degradation rate of DE-8 to rapeseed cake could reach to 81.6% after 72 h.