CAJ | 학술논문

以牦牛乳酸水解酪蛋白为氮源,在单因素试验的基础上,采用响应面设计,对巨大芽孢杆菌产青霉素 G酰化酶培养基及发酵条件进行了研究.结果表明：以葡萄糖4.28 g/L、牦牛乳酸水解酪蛋白10 g/L、苯乙酸18.43 g/L、MgCl2·6H2O 0.5 g/L、FeCl3·6H2O 0.002 g/L为培养基,在三角瓶装液量19.3%、接种量5.6%、温度28℃、时间48 h、摇床转速220 r/min的条件下,所产青霉素 G酰化酶活力为232.05 U/L,与未添加牦牛乳酸水解酪蛋白对照组相比提高了42.25%.
이모우유산수해락단백위담원,재단인소시험적기출상,채용향응면설계,대거대아포간균산청매소 G선화매배양기급발효조건진행료연구.결과표명：이포도당4.28 g/L、모우유산수해락단백10 g/L、분을산18.43 g/L、MgCl2·6H2O 0.5 g/L、FeCl3·6H2O 0.002 g/L위배양기,재삼각병장액량19.3%、접충량5.6%、온도28℃、시간48 h、요상전속220 r/min적조건하,소산청매소 G선화매활력위232.05 U/L,여미첨가모우유산수해락단백대조조상비제고료42.25%.
Taking hydrolysed yak casein acid as the nitrogen source,on basis of single-factor test,using the response surface design,the medium composition and fermenting conditions of bacillus megaterium pro-ducing penicillin G acylase were studied.The results showed that,the penicillin G acylase activity 232.05 U/Lwas obtained when medium composed of glucose 4.28 g/L,hydrolysed yak casein acid 10 g/L, phenylacetic acid 18.43 g/L,MgCl2·6H2O 0.5 g/L,FeCl3·6H2O 0.002 g/L,under this condition of liq-uid volume 19.3% in eriochrome flask,inoculation 5.6%,fermentation at 28 ℃ and 48 h as well as shaking speed 220 r/min,compared with medium of no hydrolysed yak casein acid,the penicillin G acylase activity increased 42.25%.