CAJ | 학술논문

试验选用0日龄健康爱拔益加公雏384只，采用单因子完全随机设计，共分为4个处理组，其中试验1组为基础饲粮(对照组)，试验2、3组分别以灵芝菌培养物取代对照组1%和2%的玉米，试验4组为灵芝菌培养物在能量和蛋白质水平低于对照组基础上取代3%的玉米.每个处理8个重复，每个重复12只鸡.试验期6周，分为前期和后期2个阶段，研究饲粮中添加不同水平的灵芝菌培养物对肉鸡生长性能、抗氧化性能和免疫功能的影响.结果表明：饲粮中添加灵芝菌培养物显著提高了肉鸡前、后期的血浆谷胱甘肽过氧化物酶(GSH-Px)活性(P=0.038)；降低了42日龄肉仔鸡血浆的丙二醛(MDA)含量(P=0.001).肉仔鸡前、后期的T-淋巴细胞酸性α萘酯酶染色阳性率(ANAE+)在灵芝菌培养物添加组均显著高于对照组(P≤0.001)；42日龄试验3组肉仔鸡的新城疫抗体效价(P=0.05)显著高于1、2组.第1、3组试验后期肉仔鸡的饲料转化效率显著高于第4组(P=0.041).表明在饲粮中添加灵芝菌培养物能提高肉鸡的抗氧化性能和免疫功能，并改善低营养水平上肉鸡的生长性能.
시험선용0일령건강애발익가공추384지，채용단인자완전수궤설계，공분위4개처리조，기중시험1조위기출사량(대조조)，시험2、3조분별이령지균배양물취대대조조1%화2%적옥미，시험4조위령지균배양물재능량화단백질수평저우대조조기출상취대3%적옥미.매개처리8개중복，매개중복12지계.시험기6주，분위전기화후기2개계단，연구사량중첨가불동수평적령지균배양물대육계생장성능、항양화성능화면역공능적영향.결과표명：사량중첨가령지균배양물현저제고료육계전、후기적혈장곡광감태과양화물매(GSH-Px)활성(P=0.038)；강저료42일령육자계혈장적병이철(MDA)함량(P=0.001).육자계전、후기적T-림파세포산성α내지매염색양성솔(ANAE+)재령지균배양물첨가조균현저고우대조조(P≤0.001)；42일령시험3조육자계적신성역항체효개(P=0.05)현저고우1、2조.제1、3조시험후기육자계적사료전화효솔현저고우제4조(P=0.041).표명재사량중첨가령지균배양물능제고육계적항양화성능화면역공능，병개선저영양수평상육계적생장성능.
A total of 384 0-day-old male arbor acres broilers were randomly allocated to 4 treatments with each treatment having 8 replicates of 1 2 birds.Group 1 (control group)was basal diet,group 2 and 3 were basal diet in which 1% or 2% corn was replaced equally by Ganoderma Culture (GC),group 4 con-tains 3% GC based on low energy and protein basal diet.The experiment lasted for 42 days.Supplemented with GC increased the activity of glutathione peroxidase (GSH-Px)in plasma at 21 d and 42 d (P≤0.038) and decreased the content of malondialdehyde (MDA)at 42 d(P=0.001).There was a significant increase in alphanaphthyl acetate esterase positive (ANAE+)percentage at both 21 (P=0.001)and 42 d (P<0.001)in GC addition group than that in control group.Newcastle disease (ND)antibody titers in group 3 were significantly higher than that in group 1 and 2 at 42 d(P=0.05).The feed conversation ratio in group 1 and 2 were significantly higher than that in group 4 during 22~42 d(P=0.041).The results showed that adding GC to diet could increase the antioxidant status and immune functions and improve the performance of broilers which fed with low nutritional diet.