CAJ | 학술논문

目的：体外实验研究中药骨碎补对大鼠骨髓间充质干细胞增殖分化及对Cbfα-1表达的影响。方法采用中药干预培养细胞的方法，利用骨碎补单味药物与培养基联合培养大鼠骨髓间充质干细胞（ BMSCs ），通过Ⅰ型胶原免疫组化染色检测其促BMSCs成骨分化能力，通过RT-PCR方法进行半定量分析Cbfα-1的表达，探讨中药骨碎补促成骨能力的机制。结果骨碎补药物各组Ⅰ型胶原免疫组化染色检测BMSCs成骨分化能力及RT-PCR检测Cbfα-1的表达均明显优于空白组及条件培养组（P＜0.01），0.002 g/ml药物组优于0.02 g/ml及0.0002 g/ml药物组（P＜0.05）。结论中药骨碎补可促进BMSCs增殖及成骨分化，使Cbfα1的表达加强。
목적：체외실험연구중약골쇄보대대서골수간충질간세포증식분화급대Cbfα-1표체적영향。방법채용중약간예배양세포적방법，이용골쇄보단미약물여배양기연합배양대서골수간충질간세포（ BMSCs ），통과Ⅰ형효원면역조화염색검측기촉BMSCs성골분화능력，통과RT-PCR방법진행반정량분석Cbfα-1적표체，탐토중약골쇄보촉성골능력적궤제。결과골쇄보약물각조Ⅰ형효원면역조화염색검측BMSCs성골분화능력급RT-PCR검측Cbfα-1적표체균명현우우공백조급조건배양조（P＜0.01），0.002 g/ml약물조우우0.02 g/ml급0.0002 g/ml약물조（P＜0.05）。결론중약골쇄보가촉진BMSCs증식급성골분화，사Cbfα1적표체가강。
Objective To investigate the effect of traditional Chinese medicine ( TCM) , rhizoma drynariae, on the proliferation and differentiation of rat bone marrow mesenchymal stem cells (BMSCs) and the expression of Cbf alpha1 (Cbfα-1) in vitro. Methods The method of culturing cells with the intervention of TCM was used.Rat BMSCs were cultured with the combination of single drug rhizoma drynariae and the medium.Collagen type I immunohistochemical staining was performed to detect its ability to promote the osteogenesis of BMSCs.The expression of Cbfα-1 was semi-quantitatively analyzed using RT-PCR, in order to investigate the mechanism of rhizoma drynariae promoting osteogenesis.Results The ability to promote the osteogenesis of BMSCs detected using collagen type I immunohistochemical staining and the expression of Cbfα-1 detected using RT-PCR in all rhizoma drynariae drug groups were significantly better than that in blank control group and conditioned culture group ( P<0.01 ) . The effect in the group with 0.002 g/ml of the drug was better than that in the groups with 0.02 g/ml and 0.0002 g/ml of the drug (P<0.05).Conclusion Rhizoma drynariae can promote the proliferation and osteogenesis differentiation of BMSCs and enhance the expression of Cbfα-1.