中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
20期
9037-9041
,共5页
王峰%时林森%汪灏%王萌%冯敏%管文贤
王峰%時林森%汪灝%王萌%馮敏%管文賢
왕봉%시림삼%왕호%왕맹%풍민%관문현
胃肿瘤%Gastrokine 2 基因%螺杆菌,幽门%实时荧光定量聚合酶链反应%印迹法,蛋白质
胃腫瘤%Gastrokine 2 基因%螺桿菌,幽門%實時熒光定量聚閤酶鏈反應%印跡法,蛋白質
위종류%Gastrokine 2 기인%라간균,유문%실시형광정량취합매련반응%인적법,단백질
Stomach neoplasms%Gastrokine 2 gene%Helicobacter pylori%RT-PCR%Blotting,Western
目的探讨Gastrokine 2在胃癌的发生、发展过程中的作用。方法应用实时荧光定量聚合酶链反应（RT-PCR）和Western blot印迹法检测76例诊断明确的胃癌患者的癌组织及癌旁组织中Gastrokine 2的表达，并分析胃癌组织中mRNA和蛋白的表达与临床病理特征之间的关系。结果 Gastrokine 2 mRNA和蛋白在胃癌组织中的表达均低于癌旁组织，差异有统计学意义（P＜0.01）。胃癌组织中Gastrokine 2 mRNA的表达与肿瘤的位置、浸润深度、分化程度、Lauren分型、淋巴结转移及患者的性别和年龄均无关（P＞0.01），而幽门螺杆菌（HP）阳性胃癌患者的癌组织中Gastrokine 2 mRNA表达低于HP阴性者（P＜0.01），Ⅰ期胃癌患者的Gastrokine 2 mRNA表达量高于Ⅱ、Ⅲ、Ⅳ期胃癌患者（P＜0.01）。胃癌组织中Gastrokine 2蛋白的表达与肿瘤的位置、浸润深度、分化程度、Lauren分型、淋巴结转移、TNM分期、幽门螺杆菌感染及患者的性别和年龄均无关（P＞0.01）。结论 Gastrokine 2在胃癌组织中的表达显著降低，它作为一种保护性基因在胃癌的发生和发展中扮演着重要角色，随着胃癌的进展，其抑制肿瘤的作用降低，同时HP感染可能减弱其对胃黏膜的保护作用。
目的探討Gastrokine 2在胃癌的髮生、髮展過程中的作用。方法應用實時熒光定量聚閤酶鏈反應（RT-PCR）和Western blot印跡法檢測76例診斷明確的胃癌患者的癌組織及癌徬組織中Gastrokine 2的錶達，併分析胃癌組織中mRNA和蛋白的錶達與臨床病理特徵之間的關繫。結果 Gastrokine 2 mRNA和蛋白在胃癌組織中的錶達均低于癌徬組織，差異有統計學意義（P＜0.01）。胃癌組織中Gastrokine 2 mRNA的錶達與腫瘤的位置、浸潤深度、分化程度、Lauren分型、淋巴結轉移及患者的性彆和年齡均無關（P＞0.01），而幽門螺桿菌（HP）暘性胃癌患者的癌組織中Gastrokine 2 mRNA錶達低于HP陰性者（P＜0.01），Ⅰ期胃癌患者的Gastrokine 2 mRNA錶達量高于Ⅱ、Ⅲ、Ⅳ期胃癌患者（P＜0.01）。胃癌組織中Gastrokine 2蛋白的錶達與腫瘤的位置、浸潤深度、分化程度、Lauren分型、淋巴結轉移、TNM分期、幽門螺桿菌感染及患者的性彆和年齡均無關（P＞0.01）。結論 Gastrokine 2在胃癌組織中的錶達顯著降低，它作為一種保護性基因在胃癌的髮生和髮展中扮縯著重要角色，隨著胃癌的進展，其抑製腫瘤的作用降低，同時HP感染可能減弱其對胃黏膜的保護作用。
목적탐토Gastrokine 2재위암적발생、발전과정중적작용。방법응용실시형광정량취합매련반응（RT-PCR）화Western blot인적법검측76례진단명학적위암환자적암조직급암방조직중Gastrokine 2적표체，병분석위암조직중mRNA화단백적표체여림상병리특정지간적관계。결과 Gastrokine 2 mRNA화단백재위암조직중적표체균저우암방조직，차이유통계학의의（P＜0.01）。위암조직중Gastrokine 2 mRNA적표체여종류적위치、침윤심도、분화정도、Lauren분형、림파결전이급환자적성별화년령균무관（P＞0.01），이유문라간균（HP）양성위암환자적암조직중Gastrokine 2 mRNA표체저우HP음성자（P＜0.01），Ⅰ기위암환자적Gastrokine 2 mRNA표체량고우Ⅱ、Ⅲ、Ⅳ기위암환자（P＜0.01）。위암조직중Gastrokine 2단백적표체여종류적위치、침윤심도、분화정도、Lauren분형、림파결전이、TNM분기、유문라간균감염급환자적성별화년령균무관（P＞0.01）。결론 Gastrokine 2재위암조직중적표체현저강저，타작위일충보호성기인재위암적발생화발전중분연착중요각색，수착위암적진전，기억제종류적작용강저，동시HP감염가능감약기대위점막적보호작용。
Objective To clarify the role of Gastrokine 2 in the process of formation and development of gastric cancer. Methods The expressions of Gastrokine 2 in gastric cancer and paracancerous tissues of 76 patients with gastric cancer were detected by real-time fluorescence quantitative polymerase chain reaction (RT-PCR) and Western blot. The relationship between Gastrokine 2 expression and clinico-pathologic characteristics of gastric cancer was analyzed. Results Gastrokine 2 protein and mRNA levels were reduced significantly in gastric cancer tissues compared with controls(P<0.01). Gastrokine 2 mRNA level had no remarkable correlation with tumor location, depth of invasion, differentiation, lauren, lymph node metastasis, gender and age(P>0.01, respectively), rather than TNM stage(P<0.01). In addition, Gastrokine 2 protein level also had no remarkable correlation with tumor location, depth of invasion, differentiation, lauren, TNM stage, lymph node metastasis, helicobacter pylori(HP) infection gender and age(P>0.01, respectively). However, HP infection reduced Gastrokine 2 mRNA levels significantly in gastric cancer tissues compared with controls(P<0.01). Conclusions The expression levels of Gastrokine 2 gene and protein in the gastric cancer tissues were significantly low. Gastrokine 2 may play a significant role as an anti-oncogene in the process of the formation and development of gastric cancer. Its effect may become weak as the development of tumor and HP infection in gastric cancer patients.
