中国骨质疏松杂志
中國骨質疏鬆雜誌
중국골질소송잡지
CHINESE JOURNAL OF OSTEOPOROSIS
2014年
1期
17-21,32
,共6页
基质金属蛋白酶-10%金属蛋白酶抑制物-1%骨密度%骨质疏松%绝经后
基質金屬蛋白酶-10%金屬蛋白酶抑製物-1%骨密度%骨質疏鬆%絕經後
기질금속단백매-10%금속단백매억제물-1%골밀도%골질소송%절경후
Matrix metalloproteinase 10%Tissue inhibitor of matrix metalloproteinase-1%Bone mineral density%Osteoporosis%Postmenopausal
目的：测定绝经后妇女血清基质金属蛋白酶-10（MMP-10）和金属蛋白酶抑制物-1（TIMP-1）水平，并探讨其与骨密度数值和骨代谢指标的关系。方法选取武汉地区120名48岁至65岁绝经后女性，用酶联免疫吸附试验（ ELISA ）测定血清MMP-10、TIMP-1以及整合素（Integrin）α2β1、雌二醇（E2）、骨保护蛋白（OPG）、骨保护蛋白配体（OPGL）的水平，计算MMP-10/TIMP-1比值，用双能X线吸收法（DEXA）测定腰椎正位，股骨颈，华氏区和大粗隆的骨密度（BMD）。同时，按照WHO标准将入选女性分为骨密度正常组、低骨量组和骨质疏松组。结果①骨质疏松组中血清MMP-10检测结果（644.25±19.21） pg/ml高于正常组（347.08±21.41）pg/ml （P＜0.05），而TIMP-1检测结果（134±116）μg/L低于正常组（146±130）μg/L。②骨质疏松组中血清MMP-10与骨密度、血清E2、OPGL和Integrin α2β1测定结果存在明显负相关性（ P＜0.05），和OPG和CTX存在明显正相关性（P＜0.05）。结论血清MMP-10和MMP-10/TIMP-1比值与绝经后骨质疏松症妇女骨密度和骨代谢指标具有关联性。两者可能通过整合素途径参与绝经后骨质疏松症骨代谢高转换过程。
目的：測定絕經後婦女血清基質金屬蛋白酶-10（MMP-10）和金屬蛋白酶抑製物-1（TIMP-1）水平，併探討其與骨密度數值和骨代謝指標的關繫。方法選取武漢地區120名48歲至65歲絕經後女性，用酶聯免疫吸附試驗（ ELISA ）測定血清MMP-10、TIMP-1以及整閤素（Integrin）α2β1、雌二醇（E2）、骨保護蛋白（OPG）、骨保護蛋白配體（OPGL）的水平，計算MMP-10/TIMP-1比值，用雙能X線吸收法（DEXA）測定腰椎正位，股骨頸，華氏區和大粗隆的骨密度（BMD）。同時，按照WHO標準將入選女性分為骨密度正常組、低骨量組和骨質疏鬆組。結果①骨質疏鬆組中血清MMP-10檢測結果（644.25±19.21） pg/ml高于正常組（347.08±21.41）pg/ml （P＜0.05），而TIMP-1檢測結果（134±116）μg/L低于正常組（146±130）μg/L。②骨質疏鬆組中血清MMP-10與骨密度、血清E2、OPGL和Integrin α2β1測定結果存在明顯負相關性（ P＜0.05），和OPG和CTX存在明顯正相關性（P＜0.05）。結論血清MMP-10和MMP-10/TIMP-1比值與絕經後骨質疏鬆癥婦女骨密度和骨代謝指標具有關聯性。兩者可能通過整閤素途徑參與絕經後骨質疏鬆癥骨代謝高轉換過程。
목적：측정절경후부녀혈청기질금속단백매-10（MMP-10）화금속단백매억제물-1（TIMP-1）수평，병탐토기여골밀도수치화골대사지표적관계。방법선취무한지구120명48세지65세절경후녀성，용매련면역흡부시험（ ELISA ）측정혈청MMP-10、TIMP-1이급정합소（Integrin）α2β1、자이순（E2）、골보호단백（OPG）、골보호단백배체（OPGL）적수평，계산MMP-10/TIMP-1비치，용쌍능X선흡수법（DEXA）측정요추정위，고골경，화씨구화대조륭적골밀도（BMD）。동시，안조WHO표준장입선녀성분위골밀도정상조、저골량조화골질소송조。결과①골질소송조중혈청MMP-10검측결과（644.25±19.21） pg/ml고우정상조（347.08±21.41）pg/ml （P＜0.05），이TIMP-1검측결과（134±116）μg/L저우정상조（146±130）μg/L。②골질소송조중혈청MMP-10여골밀도、혈청E2、OPGL화Integrin α2β1측정결과존재명현부상관성（ P＜0.05），화OPG화CTX존재명현정상관성（P＜0.05）。결론혈청MMP-10화MMP-10/TIMP-1비치여절경후골질소송증부녀골밀도화골대사지표구유관련성。량자가능통과정합소도경삼여절경후골질소송증골대사고전환과정。
Objective To determine the serum levels of matrix metalloproteinase-10 ( MMP-10 ) and tissue inhibitor of matrix metalloproteinase-1 ( TIMP-1) in postmenopausal women, and to explore the correlation of the serum levels of MMP-13 and TIMP-1 with the bone mineral density ( BMD) and bone metabolic markers.Methods One hundred and twenty postmenopausal women in Wuhan area, aging from 48 to 65 years old, were selected.The serum levels of MMP-10, TIMP-1, integrinα2β1, E2, OPG, and OPGL of all the subjects were detected using ELISA.And the ratio of MMP-10/TIMP-1was calculated.BMD of the lumbar vertebrae, the femoral neck, the Ward ’ s area, and the greater trochanter was detected using dual energy X-ray absorptiometry ( DEXA) .Then, all the selected subjects were divided into 3 groups according to the WHO criteria:the normal control group, the low bone mass group, and the osteoporosis group.Results The serum concentration of MMP-10 in osteoporosis group (644.25 ± 19.21 pg/ml) was significantly higher than that in normal control group ( 347.08 ±21.41 pg/ml, P<0.05 ) , while the serum concentration of TIMP-1 was lower in osteoporosis group (134 ±116 μg/L) than that in normal control group (146 ±130 μg/L). In osteoporosis group, the notable negative correlations between MMP-10, and BMD, serum E2 , OPGL, and integrin α2β1 were observed (P<0.05).Positive relations were observed between MMP-10, OPG, and CTX (P<0.05).Conclusion The serum level of MMP-10 and the ratio of MMP-10/TIMP-1 are significantly correlated with BMD and bone metabolic markers in postmenopausal osteoporosis women.They may participate in the high-speed turnover of bone metabolism in postmenopausal osteoporosis women through integrin signal pathway.
