CAJ | 학술논문

目的建立孕兔宫内窘迫模型，探讨胎兔脑一氧化氮(NO)、诱生型一氧化氮合酶(iNOS)和总超氧化物歧化酶(T-SOD)含量变化规律。方法新西兰大白孕兔30只，随机分为空白对照组(n=12)、假手术组(n=9)和宫内缺血缺氧组(n=9)，宫内缺血缺氧组建立孕兔开腹子宫动脉结扎胎兔宫内窘迫缺血缺氧模型，分别开腹取各组胎兔脑匀浆液测定NO、iNOS和T-SOD含量，进行统计学比较。结果与空白对照组比较，假手术组和宫内缺血缺氧组NO、iNOS的含量显著升高(P<0.01)， T-SOD的含量明显降低(P<0.01)，差异具有统计学意义。与假手术组相比，宫内缺血缺氧组NO、iNOS显著升高(P<0.01)，差异具有统计学意义；T-SOD降低不显著(P=0.399)。结论通过检测孕兔宫内窘迫模型NO、iNOS和T-SOD显示，胎兔缺氧应激反应强烈，手术操作及子宫动脉结扎对脑组织内自由基含量影响显著。
목적건립잉토궁내군박모형，탐토태토뇌일양화담(NO)、유생형일양화담합매(iNOS)화총초양화물기화매(T-SOD)함량변화규률。방법신서란대백잉토30지，수궤분위공백대조조(n=12)、가수술조(n=9)화궁내결혈결양조(n=9)，궁내결혈결양조건립잉토개복자궁동맥결찰태토궁내군박결혈결양모형，분별개복취각조태토뇌균장액측정NO、iNOS화T-SOD함량，진행통계학비교。결과여공백대조조비교，가수술조화궁내결혈결양조NO、iNOS적함량현저승고(P<0.01)， T-SOD적함량명현강저(P<0.01)，차이구유통계학의의。여가수술조상비，궁내결혈결양조NO、iNOS현저승고(P<0.01)，차이구유통계학의의；T-SOD강저불현저(P=0.399)。결론통과검측잉토궁내군박모형NO、iNOS화T-SOD현시，태토결양응격반응강렬，수술조작급자궁동맥결찰대뇌조직내자유기함량영향현저。
Objective To Study the changes of NO、iNOS and T-SOD in pregnant rabbits brain tissue after build acute intrauterine hypoxia model. Methods 30 New Zealand white pregnant rabbits were randomly divided into 3 groups, blank group(n=12), sham operated group(n=9) and intrauterine ischemia hypoxia group(n=9), according to uterine artery ligation and erecting uterine ischemia hypoxia models. The contents of NO, iNOS and T-SOD in pregnant rabbits brain homogenate were measured and compared. Results Compared with the blank group, the contents of NO and iNOS were significantly increased in sham operated group and intrauterine ischemia hypoxia group(P<0.01), the contents of T-SOD were significantly decreased(P<0.01). Compared with sham operated group, the contents of NO and iNOS were significantly increased in intrauterine ischemia hypoxia group(P<0.01), but the contents of T-SOD were not significantly decreased(P=0.399). Conclusion All pregnant rabbits have an strong stress response, after the operative procedure and uterine artery ligation, they have an significant effect upon the contents of NO, iNOS and T-SOD in pregnant rabbits brain tissue.