CAJ | 학술논문

目的：观察甲基泼尼松龙(methylprednisolone，MP)干预对急性期脊髓损伤凋亡相关基因bcl-2、bax、cytc和caspass-9相对表达量的影响，并探讨该药物促进脊髓功能修复的机制。方法实验动物随机分为假手术组( S组)、单纯损伤组( C组)与MP治疗组( T组)各36只，采用改良Allen’s法制作急性脊髓损伤模型，在损伤后8 h、24 h、3天、7天、14天和28天随机取各组6只动物处死，实时荧光定量PCR测量标本的ΔCt值并进行分析。结果 T组bcl-2基因ΔCt值均小于C组，差异有统计学意义(P＜0.05)；bax基因在T组术后3天、14天和28天ΔCt值大于C组，差异有统计学意义( P＜0.05)，T组8 h、24 h和7天三个时间点ΔCt值与C组，差异无统计学意义( P＞0.05)；T组cytc基因ΔCt值大于C组，差异有统计学意义( P＜0.05)；T组caspass-9基因ΔCt值大于C组，差异有统计学意义(P＜0.05)。结论甲强龙可以通过bcl-2、cytc及caspass-9的表达起到保护受伤节段脊髓的作用。
목적：관찰갑기발니송룡(methylprednisolone，MP)간예대급성기척수손상조망상관기인bcl-2、bax、cytc화caspass-9상대표체량적영향，병탐토해약물촉진척수공능수복적궤제。방법실험동물수궤분위가수술조( S조)、단순손상조( C조)여MP치료조( T조)각36지，채용개량Allen’s법제작급성척수손상모형，재손상후8 h、24 h、3천、7천、14천화28천수궤취각조6지동물처사，실시형광정량PCR측량표본적ΔCt치병진행분석。결과 T조bcl-2기인ΔCt치균소우C조，차이유통계학의의(P＜0.05)；bax기인재T조술후3천、14천화28천ΔCt치대우C조，차이유통계학의의( P＜0.05)，T조8 h、24 h화7천삼개시간점ΔCt치여C조，차이무통계학의의( P＞0.05)；T조cytc기인ΔCt치대우C조，차이유통계학의의( P＜0.05)；T조caspass-9기인ΔCt치대우C조，차이유통계학의의(P＜0.05)。결론갑강룡가이통과bcl-2、cytc급caspass-9적표체기도보호수상절단척수적작용。
Objectives To observe the effects of methylprednisolone ( MP ) on the neurocyte apoptotic genes such as bcl-2, bax, cytc caspass-9 following acute spinal cord injury ( SCI ) and investigate the mechanisms of spinal cord recovery.Methods All experimental rabbits were divided randomly into 3 groups: sham group ( group S, 36 rabbits ), control group ( group C, 36 rabbits ) and MP treatment group ( group T, 36 rabbits ). The spinal cord injury models were made according to Allen’s weight-drop method. 6 rabbits were selected randomly and sacriifced at 8 h, 24 h, 3 d, 7 d, 14 d and 28 d after the injury. The RTFQ- PCR test were taken according to their injured spinal sections, and the results of ΔCt were compared and analyzed.Results The results of ΔCt of gene bcl-2 in group T was lower than in group C, which is statistically signiifcant (P<0.05 ). At 3 d, 14 d and 28 d after the injury, the results of ΔCt of gene bax was higher in group C than in group T, which is statistically signiifcant (P<0.05 ). No signiifcant differences were found between group C and T at 8 h, 24 h and 28 d (P>0.05 ). Group T had better expressions in the ΔCt of gene cytc and caspass-9 than group C at all time points, which is statistically signiifcant (P<0.05 ).Conclusions MP plays a protective role in treating the acute spinal cord injury by affecting the expressions of the bcl-2, cytc and caspass-9 gene.