CAJ | 학술논문

目的：研究γ-促分泌酶抑制剂 MW167阻断Notch信号对脑缺血再灌注小鼠海马肿瘤坏死因子-α（TNF-α）表达的影响。方法72只昆明小鼠分为假手术组（Sham ，8只）、γ-促分泌酶抑制剂组（MW167，32只）、缺血再灌注组（I/R ，32只）。γ-促分泌酶抑制剂组和I/R组依据缺血后再灌注的不同时间点再细分为1、3、7、14 d 4个亚组。应用免疫组织化学法、蛋白印迹法检测各组小鼠海马TNF-α蛋白的表达。结果免疫组织化学和蛋白印迹法结果发现，缺血处理后的各个时间点，TNF-α表达持续增高，与假手术组相比差异有统计学意义（P＜0.01）；与相同时间点的缺血组比较，MW167组的 TNF-α蛋白阳性表达明显降低（P＜0.05）。结论调节炎性因子TNF-α蛋白的表达可能是Notch信号通路参与脑缺血再灌注损伤的机制之一。
목적：연구γ-촉분비매억제제 MW167조단Notch신호대뇌결혈재관주소서해마종류배사인자-α（TNF-α）표체적영향。방법72지곤명소서분위가수술조（Sham ，8지）、γ-촉분비매억제제조（MW167，32지）、결혈재관주조（I/R ，32지）。γ-촉분비매억제제조화I/R조의거결혈후재관주적불동시간점재세분위1、3、7、14 d 4개아조。응용면역조직화학법、단백인적법검측각조소서해마TNF-α단백적표체。결과면역조직화학화단백인적법결과발현，결혈처리후적각개시간점，TNF-α표체지속증고，여가수술조상비차이유통계학의의（P＜0.01）；여상동시간점적결혈조비교，MW167조적 TNF-α단백양성표체명현강저（P＜0.05）。결론조절염성인자TNF-α단백적표체가능시Notch신호통로삼여뇌결혈재관주손상적궤제지일。
Objective To investigate the effect of the blockage of Notch pathway by γ-secretase inhibitor MW167 on the expression of tumor necrosis factor-α (TNF-α) in hippocampus of cerebral ischemia-reperfusion mice .Methods Seventy-two kunming mice were randomly divided into the sham group (Sham ,n= 8) ,γ-se-crataseinhibitorgroup (MW167,n= 32) and ischemia-reperfusion group (I/R,n= 32).Animals from the MW167 and I/R groups were divided into subgroups based on their execution time after reperfusion (1 ,3 ,7 ,14 d) .The expression of TNF-αin hippocampus of all groups was examined by immunochemistry and Western Blot-ting .Results Compared to sham group ,the expression of TNF-αin hippocampus in MW167 and I/R group in-creased significantly at all time points after ischemia (P<0.01);at the same time point after ischemia ,the expres-sion of TNF-αin MW167 group was significantly lower than that of I/R group (P<0.05) .Conclusion One of the mechanisms of cerebral ischemia-reperfusion injury in which Notch signaling pathway was involved might be regulating the expression of TNF-α.