CAJ | 학술논문

目的：通过对类风湿关节炎（RA）患者血清白细胞介素（IL）-32、细胞间黏附分子-1（ICAM-1）、IL-10的检测，探讨IL-32、ICAM-1、IL-10在RA发病、病程发展中的意义及临床相关性。方法采用酶联免疫吸附试验（ELISA），检测115例RA、31例骨关节炎（OA）、76名对照组IL-32、ICAM-1、IL-10的水平，并与抗环瓜氨酸肽（CCP）抗体、类风湿因子（RF）、超敏C反应蛋白（hs-CRP）、红细胞沉降率（ESR）等指标进行相关性分析。结果3组比较，RA组、OA组血清IL-32、ICAM-1、IL-10浓度高于对照组，差异有统计学意义（P＜0.05）；RA重度活动期组IL-32、ICAM-1浓度显著高于缓解期组（P＜0.05）；RA组血清IL-32、ICAM-1水平与抗CCP抗体、RF、ESR、hs-CRP、28个关节疾病活动指数（DAS28）、关节肿胀数和关节压痛数呈正相关（P＜0.01），IL-10与ESR、hs-CRP呈正相关（P＜0.05或 P＜0.01）。结论 IL-32、ICAM-1、IL-10参与了RA形成，并与疾病活动程度关系密切。研究IL-32、ICAM-1、IL-10在RA病理生理过程中的生物学效应，有助于 RA发病机制的研究，为临床治疗提供依据。
목적：통과대류풍습관절염（RA）환자혈청백세포개소（IL）-32、세포간점부분자-1（ICAM-1）、IL-10적검측，탐토IL-32、ICAM-1、IL-10재RA발병、병정발전중적의의급림상상관성。방법채용매련면역흡부시험（ELISA），검측115례RA、31례골관절염（OA）、76명대조조IL-32、ICAM-1、IL-10적수평，병여항배과안산태（CCP）항체、류풍습인자（RF）、초민C반응단백（hs-CRP）、홍세포침강솔（ESR）등지표진행상관성분석。결과3조비교，RA조、OA조혈청IL-32、ICAM-1、IL-10농도고우대조조，차이유통계학의의（P＜0.05）；RA중도활동기조IL-32、ICAM-1농도현저고우완해기조（P＜0.05）；RA조혈청IL-32、ICAM-1수평여항CCP항체、RF、ESR、hs-CRP、28개관절질병활동지수（DAS28）、관절종창수화관절압통수정정상관（P＜0.01），IL-10여ESR、hs-CRP정정상관（P＜0.05혹 P＜0.01）。결론 IL-32、ICAM-1、IL-10삼여료RA형성，병여질병활동정도관계밀절。연구IL-32、ICAM-1、IL-10재RA병리생리과정중적생물학효응，유조우 RA발병궤제적연구，위림상치료제공의거。
Objective Concentrations of interleukin-32(IL-32) ,intercellular adhesion molecule-1 (ICAM-1) and interleukin-10 (IL-10) in serum of patients with rheumatoid arthritis (RA) were detected ,so to explore the relationships between the upper cytokines and the pathogenesis ,progression and effect on RA .Methods ELISA was used to detect the concentrations of IL-32 ,ICAM-1 and IL-10 in the serum of 115 patients with RA ,31 osteo-arthritis (OA) and 76 healthy control .Spearman model analysis was used to analyze the correlation of cytokines with anti-CCP antibody ,rheumatoid factor (RF) ,hypersensitive C-reactive protein (hs-CRP) and erythrocyte sed-imentation rate (ESR) .Results The concentrations of IL-32 ,ICAM-1 and IL-10 in RA and OA were significant-ly higher than that of healthy group (P<0.05) .RA severe active group and stable group in IL-32 ,ICAM-1 con-centration levels were significantly different ;IL-32 and ICAM -1 had significantly positive correlations with anti-CCP antibody ,RF ,hs-CRP ,ESR ,DAS28 ,joint swelling number and joint tenderness number (P<0.01) ,while IL-10 levels were seemly to be positive correlation with hs-CRP ,ESR (P<0.05 or P<0.01) .Conclusion IL-32 , ICAM-1 and IL-10 were likely to play important roles in RA progression ,and were closely related with RA patho-genesis .Study the biological effects of IL-32 ,ICAM-1 and IL-10 on the pathophysiological process in RA will contribute to understand the pathogenesis of RA and provide a basis for its clinical treatment .