CAJ | 학술논문

为提高生物冶金效率，研究了添加异亮氨酸对嗜酸氧化亚铁硫杆菌胞外多聚物组分、嗜酸氧化亚铁硫杆菌生长和吸附能力的影响。结果表明：与对照相比，添加异亮氨酸的嗜酸氧化亚铁硫杆菌生长周期明显缩短，Fe2＋的耗尽时间缩短，培养后期Fe2＋剩余量明显降低。最高菌密度提高17％，环境p H降低20．45％，胞外多聚物中蛋白质组分提高16．84％，多糖组分提高5．05％。培养时间为72 h时，添加异亮氨酸的嗜酸氧化亚铁硫杆菌菌液EPS吸附量为54．5208μg·mL-1，比对照提高了44．05％。整个生长周期中，异亮氨酸对菌液中游离EPS含量影响不大。
위제고생물야금효솔，연구료첨가이량안산대기산양화아철류간균포외다취물조분、기산양화아철류간균생장화흡부능력적영향。결과표명：여대조상비，첨가이량안산적기산양화아철류간균생장주기명현축단，Fe2＋적모진시간축단，배양후기Fe2＋잉여량명현강저。최고균밀도제고17％，배경p H강저20．45％，포외다취물중단백질조분제고16．84％，다당조분제고5．05％。배양시간위72 h시，첨가이량안산적기산양화아철류간균균액EPS흡부량위54．5208μg·mL-1，비대조제고료44．05％。정개생장주기중，이량안산대균액중유리EPS함량영향불대。
In order to improve the efficiency of biological metallurgy ,the effect of isoleucine added to the liquid medium on extracellular polymeric component of Acidithiobacillus ferrooxidans ,growth and adsorption capac-ity were studied .The results showed that compared with CK ,growth cycle of Acidithiobacillus ferrooxidans with isoleucine and consumption time of Fe2+ were shortened ,the highest cell density increased by 17% ,pH of medium decreased by 20 .45% ,protein content of extracellular polymeric increased by 16 .84% ,polysaccharide content increased by 5 .05% .The adsorption amount of EPS added isoleucine was 54 .520 8 μg·mL-1 for 72 h , increased by 44 .05% than CK .During the whole growth cycle ,isoleucine had little effect on dissociation of EPS .