创伤外科杂志
創傷外科雜誌
창상외과잡지
JOURNAL OF AUMATIC SURGERY
2014年
1期
42-45
,共4页
创伤%脑损伤%拉贝洛尔%肿瘤坏死因子-α%肠道通透性
創傷%腦損傷%拉貝洛爾%腫瘤壞死因子-α%腸道通透性
창상%뇌손상%랍패락이%종류배사인자-α%장도통투성
trauma%brain injury%labetalol%TNF-α%intestinal permeability
目的：探讨拉贝洛尔对创伤性脑损伤（ TBI）大鼠空肠通透性的影响。方法54只雄性Wister大鼠，随机分为对照组（n＝18）、TBI组（n＝18）、TBI＋拉贝洛尔组（n＝18）；每组伤后3、6、12h时间点各分为3个亚组（n＝6），参照Feeney’s方法建立大鼠TBI模型。在各时间点采用异硫氰酸荧光素葡聚糖（FITC-Dextran）行大鼠活体内空肠通透性评价，酶联免疫吸附剂测定法（ELISA）测定血浆肾上腺素、肠道肿瘤坏死因子-α（ TNF-α）水平，光学显微镜下观察各组肠黏膜组织形态变化。结果 TBI组大鼠在建模后各时间点血浆肾上腺素水平、肠道TNF-α水平和肠道通透性较对照组明显增加（P＜0.01），TBI＋拉贝洛尔组大鼠血浆肾上腺素水平和肠道TNF-α水平在建模后各时间点较TBI组大鼠明显降低（P＜0.01，P＜0.05），建模后6、12h TBI＋拉贝洛尔组大鼠的肠道通透性较TBI组降低（ P＜0.05）。结论拉贝洛尔能降低大鼠TBI后空肠通透性的增加。
目的：探討拉貝洛爾對創傷性腦損傷（ TBI）大鼠空腸通透性的影響。方法54隻雄性Wister大鼠，隨機分為對照組（n＝18）、TBI組（n＝18）、TBI＋拉貝洛爾組（n＝18）；每組傷後3、6、12h時間點各分為3箇亞組（n＝6），參照Feeney’s方法建立大鼠TBI模型。在各時間點採用異硫氰痠熒光素葡聚糖（FITC-Dextran）行大鼠活體內空腸通透性評價，酶聯免疫吸附劑測定法（ELISA）測定血漿腎上腺素、腸道腫瘤壞死因子-α（ TNF-α）水平，光學顯微鏡下觀察各組腸黏膜組織形態變化。結果 TBI組大鼠在建模後各時間點血漿腎上腺素水平、腸道TNF-α水平和腸道通透性較對照組明顯增加（P＜0.01），TBI＋拉貝洛爾組大鼠血漿腎上腺素水平和腸道TNF-α水平在建模後各時間點較TBI組大鼠明顯降低（P＜0.01，P＜0.05），建模後6、12h TBI＋拉貝洛爾組大鼠的腸道通透性較TBI組降低（ P＜0.05）。結論拉貝洛爾能降低大鼠TBI後空腸通透性的增加。
목적：탐토랍패락이대창상성뇌손상（ TBI）대서공장통투성적영향。방법54지웅성Wister대서，수궤분위대조조（n＝18）、TBI조（n＝18）、TBI＋랍패락이조（n＝18）；매조상후3、6、12h시간점각분위3개아조（n＝6），삼조Feeney’s방법건립대서TBI모형。재각시간점채용이류청산형광소포취당（FITC-Dextran）행대서활체내공장통투성평개，매련면역흡부제측정법（ELISA）측정혈장신상선소、장도종류배사인자-α（ TNF-α）수평，광학현미경하관찰각조장점막조직형태변화。결과 TBI조대서재건모후각시간점혈장신상선소수평、장도TNF-α수평화장도통투성교대조조명현증가（P＜0.01），TBI＋랍패락이조대서혈장신상선소수평화장도TNF-α수평재건모후각시간점교TBI조대서명현강저（P＜0.01，P＜0.05），건모후6、12h TBI＋랍패락이조대서적장도통투성교TBI조강저（ P＜0.05）。결론랍패락이능강저대서TBI후공장통투성적증가。
Objective To study the effect of labetalol on tumor necrosis factor -alpha ( TNF-α) and per-meability in rats after traumatic brain injury (TBI).Methods Fifty-four male Wister rats were randomly divided into three groups:control group(n=18),TBI group(n=18) ,TBI+labetalol group(n=18).Each group was fur-ther divided into 3 hours,6 hours and 12 hours subgroups (n=6/subgroup).The rat TBI model was established by referring to Feeney ’ s method .After the model development ,animals underwent assays of vivo intestinal permeabili-ty,evaluation of plasma epinephrine and intestinal TNF-αby ELISA method at each time point .And the pathologi-cal changes of intestinal mucosa were observed under light microscope .Results The levels of plasma epinephrine , intestinal TNF-αand permeability at each time point in TBI group were significantly higher than those in control group(P<0.01).In TBI+labetalol group,the levels of plasma epinephrine and intestinal TNF-αat each time point were significantly lower than those in TBI group (P<0.01,P<0.05).At 6 and 12 hours after model establish-ment,the level of intestinal permeability in TBI +labetalol group was significantly lower than that in TBI group ( P<0.05) .Conclusion Labetalol can decrease the levels of intestinal TNF-αand lessen the increase of intestinal per-meability of rats in TBI model .
