CAJ | 학술논문

建立嘧菌酯在荔枝上的残留分析方法，并于2011-2012年在广州和南宁进行250 g · L -1嘧菌酯悬浮剂在荔枝上的田间试验，研究其在荔枝上的消解动态和最终残留。采用甲醇提取，弗罗里硅土和中性氧化铝柱层析净化，气相色谱法（ECD）检测嘧菌酯残留量。在添加水平为0.01、0.1和1 mg · kg -1时，荔枝果肉、全果和果皮中平均添加回收率分别为83.0％～85.1％、83.3％～84.9％和80.6％～84.5％；相对标准偏差分别为2.24％～3.88％、2.77％～3.84％和2.90％～3.68％；检出限（LOD）为0.005 mg · kg -1；定量限（LOQ）为0.01mg·kg -1。田间试验结果表明，嘧菌酯在广州和南宁荔枝中的半衰期分别为2.8～5.7d和3.5～7.2d，消解迅速。250 g · L -1嘧菌酯悬浮剂，200 mg · kg -1施用3～4次，于末次施药后7 d ，荔枝全果上残留量为0.01～0.16 mg · kg -1，果肉中残留量均小于0.01 mg · kg -1，果皮中残留量为0.02～0.73 mg · kg -1；于末次施药后14 d ，全果残留量为＜0.01～0.12 mg · kg -1，果肉中的残留量均小于0.01 mg · kg -1，果皮中的残留量为0.01～0.54 mg · kg -1，全果中的最终残留量低于我国规定的M RL值0.5（mg · kg -1）。
건립밀균지재려지상적잔류분석방법，병우2011-2012년재엄주화남저진행250 g · L -1밀균지현부제재려지상적전간시험，연구기재려지상적소해동태화최종잔류。채용갑순제취，불라리규토화중성양화려주층석정화，기상색보법（ECD）검측밀균지잔류량。재첨가수평위0.01、0.1화1 mg · kg -1시，려지과육、전과화과피중평균첨가회수솔분별위83.0％～85.1％、83.3％～84.9％화80.6％～84.5％；상대표준편차분별위2.24％～3.88％、2.77％～3.84％화2.90％～3.68％；검출한（LOD）위0.005 mg · kg -1；정량한（LOQ）위0.01mg·kg -1。전간시험결과표명，밀균지재엄주화남저려지중적반쇠기분별위2.8～5.7d화3.5～7.2d，소해신속。250 g · L -1밀균지현부제，200 mg · kg -1시용3～4차，우말차시약후7 d ，려지전과상잔류량위0.01～0.16 mg · kg -1，과육중잔류량균소우0.01 mg · kg -1，과피중잔류량위0.02～0.73 mg · kg -1；우말차시약후14 d ，전과잔류량위＜0.01～0.12 mg · kg -1，과육중적잔류량균소우0.01 mg · kg -1，과피중적잔류량위0.01～0.54 mg · kg -1，전과중적최종잔류량저우아국규정적M RL치0.5（mg · kg -1）。
We developed an effective method to determine the azoxystrobin residue in lychee And field trials were designed to evaluate the dissipation and residue of azoxystrobin in lychee at Guangzhou and Nanning .The samples were extracted by methanol and detected by GC-ECD .The results showed that the average recoveries of 83.0% -85.1% ,83.3% -84.9% and 80.6% ~ 84.5% with relative standard deviations of 2.24% -3.88% ,2.77% -3.84% and 2.90% -3.68% at the spiked level of 0.01 ,0.01 and 1 mg · kg -1 .The limit of detection (LOD) was 0.005 mg · kg -1 and the limit of quantification (LOQ ) was 0.01 mg · kg -1 . Meanwhile , the half lives of azoxystrobin in lychee of Guangzhou and Nanning were 2.8 -5.7 days and 3.5 -7.2 days ,respectively ,and the terminal residue were 0.01-0.16 mg · kg -1 ,<0.01mg · kg -1 and 0.02-0.73 mg · kg -1 in the whole fruit ,flesh and peel at the 7days after last application ,and were <0.01-0.12 mg · kg -1 ,<0.01 mg · kg -1 and 0.01-0.54 mg · kg -1 in the whole fruit ,flesh and peel at the 14 days after last application .Our results suggest that the terminal residue in whole fruit are all below the maximum residue limit (MRL) set by China (0.5 mg · kg -1 ) .