CAJ | 학술논문

目的研究羧胺三唑对脂多糖诱导的 RAW264.7细胞炎症模型中炎症因子的影响。方法脂多糖（1μg/ml）刺激生长良好的RAW264.7细胞，建立细胞炎症模型，并用不同浓度羧胺三唑处理。 CCK-8法检测羧胺三唑对RAW264.7细胞的毒性作用，ELISA法检测细胞上清液中TNF-α、IL-1β和IL-6含量，分光光度法检测一氧化氮（ NO）含量。结果羧胺三唑在2.5～40μmol/L的浓度范围内对RAW264.7细胞无毒性作用（P＞0.05）。脂多糖可以明显诱导RAW264.7细胞分泌TNF-α、IL-1β、IL-6和NO（P＜0.01），10、20、40μmol/L的羧胺三唑能够显著抑制脂多糖诱导的RAW264.7细胞释放TNF-α、IL-6和NO的水平（ P＜0.05和P＜0.01），20、40μmol/L的羧胺三唑能够抑制脂多糖诱导的IL-1β的释放（ P＜0.01）。结论羧胺三唑可以抑制脂多糖诱导的RAW264.7细胞炎症反应，其抗炎作用与减少炎症因子TNF-α、IL-1β、IL-6和NO有关。
목적연구최알삼서대지다당유도적 RAW264.7세포염증모형중염증인자적영향。방법지다당（1μg/ml）자격생장량호적RAW264.7세포，건립세포염증모형，병용불동농도최알삼서처리。 CCK-8법검측최알삼서대RAW264.7세포적독성작용，ELISA법검측세포상청액중TNF-α、IL-1β화IL-6함량，분광광도법검측일양화담（ NO）함량。결과최알삼서재2.5～40μmol/L적농도범위내대RAW264.7세포무독성작용（P＞0.05）。지다당가이명현유도RAW264.7세포분비TNF-α、IL-1β、IL-6화NO（P＜0.01），10、20、40μmol/L적최알삼서능구현저억제지다당유도적RAW264.7세포석방TNF-α、IL-6화NO적수평（ P＜0.05화P＜0.01），20、40μmol/L적최알삼서능구억제지다당유도적IL-1β적석방（ P＜0.01）。결론최알삼서가이억제지다당유도적RAW264.7세포염증반응，기항염작용여감소염증인자TNF-α、IL-1β、IL-6화NO유관。
Objective To investigate the effects of carboxyamidotriazole ( CAI ) on inflammatory factors in lipopolysaccharide ( LPS)-induced RAW264.7 cells.Methods The inflammation model was established using LPS-induced RAW264.7 cell,and different concentration of CAI was added in the culture medium .The cytotoxicity of CAI was measured by cell counting kit-8 ( CCK-8 ) method.The contents of TNF-α, IL-1βand IL-6 in the culture supernatant were determined by ELISA method , and nitric oxide ( NO ) content was assayed by spectrophotometric method.Results CAI showed no cytotoxicity on RAW264.7 cells at the concentrations from 2.5 to 40μmol/L(P>0.05).LPS induced markedly increased secretions of TNF-α, IL-1β, IL-6 and NO from RAW264.7 cells ( P <0.01).CAI(10,20,40 μmol/L) exhibited the inhibitory effects on TNF-α,IL-6 and NO releases in LPS-induced RAW264.7 cells(P<0.05 and P<0.01),and CAI(20,40 μmol/L)inhibited LPS-induced IL-1βrelease(P<0.01 ) .Conclusion CAI has anti-inflammtory effects , which might be mediated by decreasing the inflammatory factors such as TNF-α,IL-1β,IL-6 and NO.