大理学院学报:综合版
大理學院學報:綜閤版
대이학원학보:종합판
Journal of Dali University
2012年
3期
5-8
,共4页
甾体皂苷%肠内菌群%代谢物%液-质联用法
甾體皂苷%腸內菌群%代謝物%液-質聯用法
치체조감%장내균군%대사물%액-질련용법
steroidal saponins%intestinal flora%metabolism%LC/MS/MS
目的:观察大鼠肠内菌对知母主要活性成分甾体皂苷的代谢及对相应代谢产物进行鉴定。方法:离体培养大鼠肠道菌群,用LC/MS/MS法检测离体肠内菌对甾体皂苷样品的代谢产物。灌胃给予大鼠甾体皂苷样品15 mg.kg-1,于给药后不同时间采集尿样及粪样,用LC/MS/MS法检测甾体皂苷样品的代谢产物。对主要代谢物M1进行结构鉴定。用合成的方法制备代谢物M1标准品进行LC/MS/MS法分析并与大鼠离体和在体样品对比。结果:甾体皂苷样品容易被大鼠消化道菌群代谢,代谢速率很快,其主要代谢产物为代谢物M1,随着代谢时间的延长,代谢物M1的量逐渐增多,而甾体皂苷样品的量则下降迅速。结论:甾体皂苷可被大鼠肠内菌迅速代谢。
目的:觀察大鼠腸內菌對知母主要活性成分甾體皂苷的代謝及對相應代謝產物進行鑒定。方法:離體培養大鼠腸道菌群,用LC/MS/MS法檢測離體腸內菌對甾體皂苷樣品的代謝產物。灌胃給予大鼠甾體皂苷樣品15 mg.kg-1,于給藥後不同時間採集尿樣及糞樣,用LC/MS/MS法檢測甾體皂苷樣品的代謝產物。對主要代謝物M1進行結構鑒定。用閤成的方法製備代謝物M1標準品進行LC/MS/MS法分析併與大鼠離體和在體樣品對比。結果:甾體皂苷樣品容易被大鼠消化道菌群代謝,代謝速率很快,其主要代謝產物為代謝物M1,隨著代謝時間的延長,代謝物M1的量逐漸增多,而甾體皂苷樣品的量則下降迅速。結論:甾體皂苷可被大鼠腸內菌迅速代謝。
목적:관찰대서장내균대지모주요활성성분치체조감적대사급대상응대사산물진행감정。방법:리체배양대서장도균군,용LC/MS/MS법검측리체장내균대치체조감양품적대사산물。관위급여대서치체조감양품15 mg.kg-1,우급약후불동시간채집뇨양급분양,용LC/MS/MS법검측치체조감양품적대사산물。대주요대사물M1진행결구감정。용합성적방법제비대사물M1표준품진행LC/MS/MS법분석병여대서리체화재체양품대비。결과:치체조감양품용역피대서소화도균군대사,대사속솔흔쾌,기주요대사산물위대사물M1,수착대사시간적연장,대사물M1적량축점증다,이치체조감양품적량칙하강신속。결론:치체조감가피대서장내균신속대사。
Objective: To study the metabolism of steroidal saponins extracted from Anemarrhena asphodeloides in rat intestinal flora in vitro and to identify the major metabolite.Methods: Rat intestinal flora and steroidal saponins extractions were incubated in vitro,and LC/MS/MS method was used to examine metabolites of steroidal saponins.The urine samples and dungs of the rats were collected after the rats were given with steroidal saponins extract in dose of 15 mg·kg-1 by gavage.The metabolites of the steroidal saponins were detected with LC/MS/MS,and the structure of the major metabolite M1 was identified.Meanwhile,the M1 obtained from synthesis was compared with the natural M1 both in vitro and in vivo.Results: In vitro,steroidal saponins extracted from A.asphodeloides were metabolized easily by rat intestinal flora,and the metabolic rate was very quickly,and the major metabolite was M1.The metabolite M1 increased gradually with the prolongation of incubation time,but the quantity of steroidal saponins extract were dropped quickly.Conclusion: Steroidal saponins extracted from A.asphodeloides are metabolized easily by rat intestinal flora.